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核糖体蛋白S6对果蝇发育的影响 被引量：1

Effect of Ribosomal Protein S6 on Drosophila Development
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摘要 核糖体蛋白S6在细胞周期和细胞凋亡等过程中发挥着重要作用.通过对RNAi干扰S6基因表达的果蝇与S2细胞表型的观察,分析细胞增殖与细胞周期和细胞凋亡相关基因的变化,结果发现:S6基因表达干扰引起果蝇幼虫、眼睛、翅膀和刚毛发育异常,并阻滞S2细胞正常生长;在果蝇翅膀discs中有凋亡信号出现;细胞周期和细胞凋亡相关基因异常表达.说明S6蛋白表达量减少引起的果蝇异常表型主要是通过细胞凋亡方式发生的. Ribosome protein S6 plays an important role in the process of cell cycle and apoptosis. We depleted S6 gene through RNA interference（ RNAi） in Drosophila melanogaster and S2 cell,respectively. By observing the phenotype changes,analyzing cell proliferation,the change of cell cycle and apoptosis related genes,the results showed that depletion of S6 gene caused the the abnormal development of Drosophila larva,eyes wings and bristles,and arrested the S2 cell normal growth; apoptotic signals appeared in the wing discs; there was the abnormal expression of genes related with cell cycle and apoptosis. These data indicated that reducing the expression of S6 protein caused the Drosophila abnormal phenotype,which was achieved by apoptosis.
作者 李红艳
机构地区 西藏民族学院医学院
出处 《华南师范大学学报（自然科学版）》 CAS 北大核心 2014年第3期107-111,共5页 Journal of South China Normal University(Natural Science Edition)
基金 科学技术部973计划(2011CB965300)
关键词 核糖体蛋白 S6蛋白 RNAI 凋亡 果蝇 ribosomal protein S6 protein RNAi apoptosis Drosophila
分类号 Q785 [生物学—分子生物学]
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参考文献33

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华南师范大学学报（自然科学版）
2014年 第3期

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