纳米介孔生物活性玻璃的制备及研究

Study and Preparation of Nano-mesoporous Bioactive Glass

本文用模板法制备了一种纳米介孔生物活性玻璃,通过动态光散射和电镜对其进行了表征。同时研究了这种纳米介孔生物活性玻璃在模拟体液中的生物活性,实验结果用小角X射线衍射,傅里叶变换红外光谱和扫描电镜表征,确定这种纳米介孔生物活性玻璃在模拟体液48小时以后就能形成羟基磷灰石结晶的沉积,同时电镜观察纳米颗粒出现了团聚。用3-氨丙基-三乙氧基硅烷(APTES)将这种生物玻璃表面氨基化后,将胶原固定其上,通过细胞培养确定胶原的固定能够有效的提高细胞在这种生物活性玻璃表面的生长情况。

In this paper,a nano-mesoporous bioactive glass was prepared by using cetyl trimethyl ammonium bromide （CTAB） as the model agent. The properties of the bioactive glass were characterized by dynamic light scattering （DLS） and electron microscope （including SEM and TEM）. Bioactivities of the bioglass were performed in the simulat-ed body fluid （SBF）. The results of wide-angle X-ray diffraction （WAXD）,Fourier transformation infrared spectrosco-py （FTIR） and scanning electron microscope （SEM） indicated that after immersing in SBF over 48 hours,the particles aggregated together. Collagen was selected to immobilize on the surface of nano-mesoporous bioactive glass modified by 3-aminopropyltriethoxysilane （APTES）. Furthermore,cells were incubated on the surface of collagen immobilized na-no-mesoporous bioactive glass to evaluate cell adhesion and proliferation.