RP-HPLC法测定保婴丹中麻黄碱的含量

RP-HPLC Determination of Ephedrine in Baoying Dan

目的建立保婴丹中麻黄碱的含量测定方法，为更好地评价保婴丹质量提供依据。方法采用高效液相色谱（HPLC）法，以Venusil MP C18（5μm，250mm×4．6mm）为色谱柱；流动相：乙腈-0．02m0L／L磷酸二氢钾溶液（用磷酸调pH值为2．35）（5：95）为流动相：检测波长为210nm；柱温为25℃。结果麻黄碱在0．0238～0．597μg（r=1．0000）范围内呈良好线性关系；低中高3个水平的平均加样回收率分别为100．3％，RSD为0．8％（n=3）；99．1％，RSD为0．7％（n=3）；101．0％，RSD为1．0％（n=3）。结论该方法简便、快速、准确、重复性好，可用于保婴丹质量控制。

Objective To establish a method for determining basis for its quality control. Methods The HPLC method was the content of ephedrine in Baoying Dan, and to provide adopted, the separation was performed on a column of Venusil MP C18 （5μm,250mm×4.6mm）, acetonitrile - 0.02 mol/L potassium dihydrogen phosphate solution （5 ： 95 ） （adjusted to pH 2.35 with phosphoric acid） was used as the mobile phase, the detection wavelength was 210 nm with the column temperature of 25 ℃. Results The ephedrine showed a good linearity in the range of 0. 023 8 - 0. 597 μg （ r = 1. 000 0） ; the average recoveries of components at three concentration levels were： 100.3% , RSD = 0.8% （n = 3） ; 99.1%, RSD=0.7% （n =3）; 101.0%, RSD =1.0% （n =3）. Conclusion The method is simple, quick, accurate, and has good reproducibility, it can be used for the quality control of Baoying Dan.