不同盐度下半滑舌鳎幼鱼非特异性免疫酶活力分析

Analysis of the Activity of Non-Specific Immune Enzymes of Juvenile Tongue Soles Cultured in Various Salinities

让半滑舌鳎幼鱼(Cynoglossus semilaevis)在淡水、盐度5、10、20和30条件下适应60d,研究幼鱼不同组织(肌肉、肝脏、鳃、肾脏)中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、碱性磷酸酶(AKP)和酸性磷酸酶(ACP)的活力。结果表明:半滑舌鳎幼鱼在淡水中生长受到了明显的抑制(P<0.05),末体重在盐度10和20时较高;半滑舌鳎幼鱼肌肉中SOD活力在淡水条件下显著高于其它各处理(P<0.05),肝脏中SOD的活力在盐度20时活力出现最大值(P<0.05),在盐度30时,鳃和肾脏中SOD活力显著高于盐度5和10时(P<0.05);肝脏中CAT活力随实验盐度的升高而降低,在淡水条件下要显著高于其它盐度(P<0.05);肌肉和肾脏中AKP活力随实验盐度的升高而逐渐升高,其中肌肉组织中AKP活力在盐度20和30时显著高于淡水处理(P<0.05);在淡水和盐度5时,肝脏中ACP活力显著高于盐度20和30时(P<0.05),鳃和肾脏ACP活力则在盐度5时较低。综上表明,本研究规格下半滑舌鳎幼鱼不适宜在淡水条件下养殖,但盐度5以上的低盐度则不影响其正常生长;不同盐度对半滑舌鳎幼鱼的非特异性免疫酶活力影响显著,且盐度对不同组织中非特异性免疫酶活力影响存在一定的组织特异性。

Effect of various salinities （0, 5, 10, 20 and 30 ppt ） on the growth of juvenile tongue sole （Cynoglossus semilaevis） and the activity of their non-specific immune enzymes （superoxide dismutase, SOD; catalase, CAT; alkaline phosphate, AKP; acidic phosphate, ACP） in different tissues （liver, gills, muscle and kidney） were investigated in this study. The results showed that the growth of juvenile tongue sole was inhibited significantly at 0 ppt （P〈0.05）. The final weight of juvenile fish was higher at intermediate salinity （10 and 20 ppt） than at 30 ppt. At 0 ppt, the activity of SOD in muscle was significantly higher than at other sanilities. The highest activity of SOD was found in liver when the salinity was 20 ptot, which was significantly higher than at 0 ppt （P〈0.05）. CAT activity in liver decreased with the increase of salinity, and that at 0 ppt was significantly higher than at other sanilities （P〈0.05）. AKP activity in renal and muscular increased with the increase of salinity. AKP activity in muscle was found to increase significantly at salinities higher than 0 （P〈0.05）. ACP activity in liver at 0 and 5 ppt was significantly higher than those at 20 and 30 ppt. The lowest ACP activity was found in gills and kidney when salinity was 5 ppt. Our findings indicated that juvenile tongue sole were not suitable for fresh water culture; however, they were suitable for salinities higher than 5 ppt. Therefore, change in salinity had a significant effect on the activity of nonspecific immune enzymes of juvenile tongue sole and such effects were different among tissues.