2012年我国部分地区猪瘟流行病学调查及E2部分基因遗传演化分析

Epidemiological Survey of Classical Swine Fever in Partial Areas of China in 2012 and Genetis Analysis of E2 Gene

为分析我国猪瘟病毒(CSFV)遗传变异情况,根据已发表的文献设计合成E2主要抗原区基因的引物,采用RT-PCR方法,对2012年我国12个省收集到的组织样品进行E2基因扩增与序列测定,并进行遗传进化分析。结果表明,在分离到的107株猪瘟阳性样品中,48株属于基因Ⅰ群中的1.1亚群,占45%;59株属于基因Ⅱ群中的2.1b和2.1c亚群,占55%,未发现传统流行亚群2.2与2.3。表明我国目前流行的CSFV主要是1.1和2.1亚群。分析发现,2012年分离的48株的1.1亚群病毒均是弱毒,与HCLV毒株亲缘关系较近,而与Shimen毒株较远,表明2.1亚群是我国目前CSFV流行的主要基因亚群,其中2.1b是CSFV流行的优势毒株,2.1c毒株有不断扩散的趋势。

To investigate the variation of Classical swine fever virus （CSFV） in China, samples collected in 2012 from 12 provinces were positive in classical fever virus detected by RT-nested PCR and sequenced. To gain insight into the molecular epidemiology of CSFV in China, we analyzed E2 gene of 107 isolates, The result revealed that all of these 107 isolates belonged to subgroup 1. land 2. 1. There were 48 isolates （45 of total） classified in subgenotype 1.1, and 59 isolates （55 ~ of total） classified in subgenotype 2.1, and none of isolates were classified in subgenotype 2.2 or subgenotype 2.3. All viruses of subgenotype 1.1 were close to HCLV strain and far from Shimen strain. So they were presumed to be lentogenic strains. Most of viruses belonged to 2.1. The isolates of 2. lb were more prevalent than others and the viruses of 2. lc had a tendency to be popular.