猪腹泻病例中PEDV、TGEV及PRV的感染调查

Investigation on Porcine Epidemic Diarrhea Virus,Transmissible Gastroenteritis Virus and Pseudorabies Virus in Swine Diarrhea Cases

近几年来,仔猪腹泻给我国养猪业造成了很大的损失,其病因至今存在争议。为了解临床腹泻病例中猪流行性腹泻病毒(PEDV)、传染性胃肠炎病毒(TGEV)及伪狂犬病病毒(PRV)的感染情况,本研究采用PCR方法对在2012年6月至2013年5月间采集的219份来源于腹泻病例的粪便样品中PEDV、TGEV及PRV进行了检测。结果表明,病毒检测阳性率为PEDV 17.35%,PRV 7.76%,PEDV+PRV0.91%,TGEV 0;对不同年龄段的猪群进行统计,哺乳仔猪PEDV阳性率最高,为25.8%,保育-育肥猪PRV阳性率最高,为12.33%。对发病时间统计,发现PEDV在2013年4月阳性率最高,为14例;PRV在2012年9月发病率最高,共检出8例。在19个城市的送检样品中,9个城市有PEDV感染发生,有8个城市有PRV感染发生,有6个城市的样品中发现混合感染。说明PEDV可能是引起这些腹泻病例的主要病原。

In recent years,porcine diarrhea has caused great economic losses in China.By now,the causes of the disease are still controversial.To investigate the distribution of several related pathogens,including porcine epidemic diarrhea virus （PEDV）,transmissible gastroenteritis virus （TGEV）and pseudorabies vi-rus （PRV）in diarrhea pigs,a total of 219 fecal samples were collected from June,2012 to May,2013 and PCR methods were used to detect the related viruses.The result showed that 17.35% and 7.76% samples were positive for PEDV and PRV,respectively,none of TGEV was detected.The percentage of both PEDV and PRV positive samples was 0.91%.The highests incidence of PEDV （25.8%）and PRV （12.33%）are found in piglets and nursery-finishing herds,respectively.The highest positive rate of PEDV was detected in April,2013 and the highest incidence of PRV was occurred in September,2012.A-mong the 19 cities,PEDV were detected in 9 cities,and PRV were found in 8 cities,mixed infections were detected in 6 cities.The results indicated that PEDV might be one of the major causes of the diarrhea in pigs.