骨髓间充质干细胞对Ⅰ型糖尿病大鼠淋巴细胞增殖的调节作用

Regulation of bone marrow mesenchymal stem cells on lymphocyte proliferation of type Ⅰ diabetic rats

目的:探讨骨髓间充质干细胞抑制Ⅰ型糖尿病大鼠淋巴细胞增殖的免疫调节相关机制。方法:分离、培养并鉴定大鼠的骨髓间充质干细胞,采用MTT法观察其对Ⅰ型糖尿病大鼠淋巴细胞增殖能力的影响,采用流式细胞仪分析骨髓间充质干细胞对Ⅰ型糖尿病大鼠CD4+CD25+调节性T细胞比例、细胞周期和细胞凋亡的影响。结果:B组和C组的吸光度值明显低于A组,数据之间比较差异均有统计学意义(P<0.05),C组吸光度值明显低于B组,数据之间比较差异有统计学意义(P<0.05);B组和C组的CD4+CD25+调节性T细胞比例均明显高于A组,数据比较差异均有统计学意义(P<0.05),C组CD4+CD25+调节性T细胞比例明显高于B组,数据比较差异有统计学意义(P<0.05);B组和C组的细胞凋亡水平明显高于A组,数据比较差异有统计学意义(P<0.05),C组细胞凋亡水平明显高于B组,数据比较差异有统计学意义(P<0.05)。结论:骨髓间充质干细胞可以显著抑制Ⅰ型糖尿病大鼠淋巴细胞增殖,其机制可能通过调节CD4+CD25+调节性T细胞比例,促进细胞凋亡,继而影响T淋巴细胞的免疫功能,发挥其抑制能力。

Objective：To investigate the regulation mechanisms of the bone marrow mesenchymal stem cells on lymphocyte pro -liferation of type I diabetic rats .Methods：The rat bone marrow mesenchymal stem cells were isolated , cultured and identified and the effect on lymphocyte proliferation of type Ⅰdiabetic rat was observed by MTT assay , and analyze the CD 4^＋CD25^＋regulatory T cell ra-tio, cell cycle and apoptosis of type I diabetes rat by flow cytometric .Results：B and C groups was significantly lower than the absor-bance values of group A,the differences between the data were statistically significant （P〈0.05）, C group was significantly lower than group B absorbance values, the difference was significant （P〈0.05）;the CD4^＋CD25^＋regulatory T cells of B and C groups were sig-nificantly higher than group A, the differences of the data were statistically significant （P〈0.05）, the CD4^＋CD25^＋regulatory T cell ratio of C group significantly higher than that group B , the differences were statistically significant （P〈0.05）;the apoptosis levels of B and C groups were significantly higher than group A , the differences were statistically significant （P〈0.05）, the apoptosis levels of C group were significantly higher in group B , the differences were statistically significant （P〈0.05）.Conclusion：Bone marrow mesen-chymal stem cells can significantly inhibit lymphocyte proliferation of type Ⅰdiabetic rats, and it may regulate CD4^＋CD25^＋regulatory T cells, promote apoptosis, thereby affecting the immune function of T lymphocytes , and play its rejection.