兰州地区2411例孕妇羊水细胞染色体核型产前诊断分析

Analysis on karyotype of amniotic fluid cells from 2411 fetus in Lanzhou

目的探讨羊水细胞培养染色体核型分析技术在细胞遗传学产前诊断中的应用及意义。方法2411例妊娠17～26周的孕妇在超声引导下行羊膜腔穿刺术，进行细胞培养及染色体核型分析。结果2411例标本一次性培养成功2391例，培养成功率为99．2％。共检出异常核型93例，异常率为3．89％，其中21-三体40例，18-三体11例，其他异常核型42例。结论羊水细胞学检查作为一项产前诊断技术对于指导优生优育，降低缺陷儿的出生具有重要意义。

Objective： To investigate the significance of chromosome karyotype analysis of amniotic fluid cells in prenatal diagnosis. Methods： Amniocentesis by guided B-ultrasound was performed on 2411 cases of pregnant women. Chromosome karyotypes from amniotic fluid samples were then analyzed. Results： 2391 cells were cultured successfully, and the success rate of primary culture was 99.2%. 93 abnormal karyotypes were detected, including 40 21-trisomes, 11 18-trisomes. Conclusion： Chromosome karyotype analysis of amniotic fluid ceils has zreat siznificance in orenatal diagnosis.