UPLC-MS法同时测定大鼠血浆中氧化芍药苷、没食子酸及咖啡酰基奎宁酸类成分

Simultaneous determination of hydroxyl-paeoniflorin, gallic acid and dicaffeoylquinic acid in rat plasma by UPLC-MS

目的建立UPLC-MS法同时测定大鼠静脉注射辛芍（灯盏细辛和赤芍）提取物后血浆中氧化芍药苷、没食子酸、1,3-二咖啡酰基奎宁酸、3,4-二咖啡酰基奎宁酸、4,5-二咖啡酰基奎宁酸5种成分的分析方法。方法血浆样品酸化后经乙腈沉淀除去蛋白,UPLC分析采用Waters Acquity BEH C18色谱柱,流动相为0.1%甲酸乙腈-0.1%甲酸水梯度洗脱,采用电喷雾电离源,扫描方式为选择性离子监测模式。结果 5种成分在血浆中线性关系良好,提取回收率91.95%～109.09%,日内、日间精密度和准确度良好,被测成分在大鼠体内消除较快。结论本分析方法特异、快速、准确灵敏,可用于氧化芍药苷、没食子酸、1,3-二咖啡酰基奎宁酸、3,4-二咖啡酰基奎宁酸、4,5-二咖啡酰基奎宁酸的药代动力学研究。

AIM To develop a UPLC-MS method for simultaneously determing the contents of hydroxyl-paeoniflorin,gallic acid,1,3- dicaffeoylquinic acid,3,4- dicaffeoylquinic acid,and 4,5- dicaffeoylquinic acid after intravenous administration of Xinshao extract（ Erigerontis Herba,Paeoniae Radix rubra） in rat plasma. METHODS Following a protein precipitation with acidified acetonitrile for plasma samples. The UPLC analysis was performed on Waters Acquity BEH C18column and the mobile phase consisting of acetonitrile and water（ containing 0. 1% formic acid） in gradient elution manner. The compounds were ionized in the electrospray ionization ion source of the mass spectrometer and detected in the selective ion monitoring mode. RESULTS Good linearity was achieved for the five compounds,the extraction recovery was between 91. 95% to 109. 09,the intra-and inter-day precisions and the accuracy were good. The five compounds were eliminated in vivo rapidly. CONCLUSION The method is specific,sensitive and suitable for the pharmacokinetic studies of the five compounds.