肝细胞癌患者调节性T细胞对CD8＋T淋巴细胞凋亡的影响

Effects of regulatory T cell cells of patients with hepatocellular carcinoma on apoptosis of CD8 ＋ T lymphocytes

目的研究肝细胞癌患者调节性T细胞（Treg）对CD8＋T淋巴细胞凋亡的影响。方法采集20例肝细胞癌患者和20例正常人的外周血，免疫磁珠分离法分别分离肝细胞癌患者Treg和正常人Treg及CD8＋T淋巴细胞，用流式分析法分别检测与肝细胞癌患者Treg及正常人Treg培养72h后CD8＋T淋巴细胞的凋亡情况。结果与正常人Treg共培养72h后，CD8＋T淋巴细胞凋亡率为（78．2±5．79）％，与肝细胞癌患者Treg共培养72h后CD8＋T淋巴细胞凋亡率为（93．2±4．87）％，两者比较有统计学意义（t=7．54，P〈0．01）。结论肝细胞癌患者免疫抑制性Treg可使CD8＋T淋巴细胞的凋亡率增加。

Objective To study the influence regulatory T cells （Treg） of patients with hepatocellular carcinoma（ HCC ） on the apoptosis of CD8 ＋ T lymphocytes. Methods Treg cells from healthy subjects and HCC patients by magnetic bead cell sorting（MACS）, mixed with CD8 ＋T lymphocytes obtained from healthy subjects cultured 72 hours. Apoptosis of CD8 ＋ T lymphocytes was detected by flow cytometry. Results Apopto- sis of CD8 ＋ T lymphocytes cultured with Treg cells from HCC patients（ 93.2± 4. 87 ） % were significantly in- creased compared with those cultured with Treg cells from healthy subjects （78, 2 ± 5.79） % （ t = 7.54, P 〈 0. 01 ）. Conclusion The Apoptosis of CD8 ＋ T lymphocytes was significantly increased by Trez cells.