Pb胁迫下接种丛枝菌根真菌对茶树解毒能力的影响

Effects of Arbuscular Mycorrhizal Fungi(AMF) Inoculation on Detoxification Capacity of Tea Tree under Pb Stress

采用盆栽试验研究了接种丛枝菌根真菌(Arbuscular mycorrhizal fungi,AMF)对铅(Pb)胁迫下茶树解除Pb毒性能力的影响,分别用醋酸墨水染色法、考马斯亮蓝法、电感耦合等离子体(ICP-MS)、紫外分光光度法等方法研究了接种AMF后茶树根系菌丝的侵染率,土壤中球囊霉素(Glomalin-related soil protein,GRSP)含量,茶树根系、叶片、土壤中及GRSP中Pb含量及叶片的抗氧化酶活性.结果表明,接种AMF后,茶树根系菌丝侵染率为60.13%,茶树的生物量、百芽重和显著增加,并极显著地提高土壤中总GRSP含量,由于GRSP对Pb的大量螯合作用,使得土壤中Pb的生物有效性大大降低,加之茶树根系、叶片中对Pb的总吸收量减少,且根系Pb含量增加,因此,叶片中Pb含量显著下降,抗氧化酶活性也相应地下降.

Potted tea trees were inoculated with arbuscular mycorrhizal fungi（Glomus intraradices） under Pb stress to investigate the effects of inoculation on detoxification apacity of tea tree. Methods including acetate ink staining,Coomassie brilliant blue,inductively coupled plasma（ICP-MS） and UV spectrophotometry method respectively were used to determine tea tree root colonization rate of mycelium,content of Glomalin-related soil protein（GRSP）,Pb content in tea tree roots,leaves,soil and GRSP,and antioxidant enzyme activity of leaves. The results showed that AMF inoculation rate in tea tree roots was 60.13%,tea tree biomass and 100-bud weight increased significantly. The total GRSP content also increased remarkably after inoculation with AFM. For GRSP chelated Pb in soil,the bioavailability of Pb in soils reduced greatly. For the total contents of Pb in roots and leaves decreased,and more Pb deposited in roots after inoculation,the Pb contents in leaves decreased significantly, and the antioxidant enzyme activity correspondingly reduced.