蒙药金色诃子炮制品对α-萘异硫氰酸酯致大鼠肝损伤的保护作用及其急性毒性研究

Study on Protective Effect of Processed Products of Traditional Mongolian Herbal Medicine Terminalia Chebula Retz. on Rats Liver Injury Induced by α- Naphthyl Isothiocyanate and Acute Toxicity

目的:观察蒙药金色诃子不同炮制品对α-萘异硫氰酸酯致大鼠肝损伤的保护作用及其急性毒性。方法:采用a-萘异硫氰酸酯建立大鼠肝损伤模型,给予不同金色诃子炮制品,观察其降酶保肝作用。采用最大给药量方法进行急性毒性实验。结果:与模型组比较,烘制金色诃子能够明显降低a-萘异硫氰酸酯所致肝损伤大鼠血清AST和ALT水平(P<0.05)。急性毒性实验小鼠未见异常反应,烘制金色诃子、煮制金色诃子、金色诃子肉最大给药剂量为临床用药量的500倍,生品最大给药剂量为临床用药量的375倍。结论:烘制金色诃子对a-萘异硫氰酸酯所致急性肝损伤具有降酶保肝作用,毒性较小,较为安全。

Objective：To observe the protective effect of Terminalia Chebula Retz.processed prodoct by different methods on liver injuries induced by α-Naphthyl isothiocyanate in rats and acute toxicity test were acted.Method：α-Naphthyl isothiocyanate were used to induce rats＇ liver injury to build models and processed products of Terminalia Chebula Retz.in different methods were given to rats for 7 days,then observing the levels of serum AST and ALT.The maximum dose method was used to research the acute toxicity.Results：To compared with model group,baked Terminalia Chebula Retz.could reduce serum AST and ALT contents of rats with liver injured significantly,（P ＜ 0.05）,no abnormal reaction was observed after 7 days during acute toxicity test.The maximum dose of baked,boiled and pulp of Terminalia Chebula Retz.was 500 fold of clinical usage,crude herbal was 375 fold of clinical usage.Conclusion：Baked Terminalia Chebula Retz can reduce serum AST and ALT levels of rats with liver injury induced by α-Naphthyl isothiocyanate,the toxicity was low,safe relatively.