三种不同中药单体对HaCaT细胞过度增殖模型的影响

Effects of catalpol, L-shikonin and paeonol extracted from radix rehmanniae, radix arnebiae and cortex moutan on KGF-induced HaCaT cell proliferation

目的 探讨地黄、紫草、丹皮3种不同中草药所含药效成分对HaCaT细胞增殖的影响.方法 采用10 μg/L角质形成细胞生长因子诱导HaCaT细胞过度增殖模型.运用CCK-8法及流式细胞仪分别检测梓醇、左旋紫草素、丹皮酚对模型细胞的增殖和细胞周期的变化.结果 左旋紫草素（≥10-6moL/L）和丹皮酚（≥1.88×10-4mol/L）均可抑制细胞模型的增殖,梓醇（≥10-6moL/L）可促进细胞模型的增殖.与HaCaT细胞生长因子模型组比较,左旋紫草素（≥10-6mol/L）和丹皮酚（≥1.88×10-4moL/L）可抑制HaCaT细胞进入S期及G2M期,梓醇（≥10-6mol/L）可促使HaCaT细胞进入S期,但G2M期差异无统计学意义.结论 左旋紫草素、丹皮酚能够抑制HaCaT细胞的增殖,梓醇可促进HaCaT细胞的增殖.

Objective To explore the effects of Chinese herbal medicine radix rehmanniae,radix arnebiae and cortex moutan on the proliferation of HaCaT cells and explore their potential curative mechanisms.Methods The main monomers of catalpol,1-shikonin and paeonol were extracted.And 10 ng/ml keratinocyte growth factor （KGF） was used to induce HaCaT cell to build an in vitro model of hyperproliferation of epidermal keratinocytes.CCK-8 assay and flow cytometry were applied to examine the effects of herbal monomers on cell proliferation and cell cycle.Results Both 1-shikonin （≥ 10-6 mol/L） and paeonol （≥ 1.88 × 10-4 mol/L） inhibited cell proliferation while catalpol（≥ 10-6 mol/L）enhanced cell proliferation.L-shikonin （≥10-6 mol/L） and paeonol （≥ 1.88 × 10-4 mol/L） inhibited the HaCaT cell during S and G2M phases while catalpol （≥ 10-6 mol/L） enhanced HaCaT cell during S phase but not G2M phase.Conclusion L-shikonin and paeonol inhibits the proliferation of HaCaT cells while catalpol has opposite effects.