亲环素A在类风湿关节炎患者滑膜成纤维细胞中的表达

Expression and significance of cyclophilin A in synovial fibroblasts from patients with rheumatoid arthritis

目的 观察类风湿关节炎（RA）患者滑膜成纤维细胞（SF）亲环素A （CyPA）的表达水平及脂多糖（LPS）对其CyPA表达的影响,探讨CyPA在RA发病及炎症活动中的可能作用.方法 所有滑膜组织取自2013年4至10月在中山大学附属第三医院及广东省第二人民医院行膝关节手术患者,包括12例RA、9例骨关节炎（OA）患者及5例关节创伤（Trauma）患者,分离并培养SF.分别通过蛋白质印迹（WB）与实时定量-聚合酶链反应（RT-qPCR）检测SF CyPA蛋白与mRNA,将RA及OA组SF CyPA蛋白水平与其临床指标作相关分析,并观察LPS对3组SF CyPA表达的影响.结果 RA组SF CyPA蛋白与mRNA的相对定量值分别为0.86±0.47与0.54±0.22,明显高于OA组（0.40±0.31与0.03 ±0.02,P＜0.05）及Trauma组（0.34±0.21与0.03±0.01,P＜0.05）.RA患者SFCyPA蛋白相对定量值与红细胞沉降率、类风湿因子及肿胀关节数均存在正相关（P＜0.05）.经LPS处理后RA患者SF CyPA蛋白与mRNA的相对定量值分别为2.65±1.16与1.82±0.39,明显高于未经LPS处理者（P＜0.05）,而OA及Trauma患者SF CyPA表达经LPS处理后未出现有统计学意义的变化.结论 RA患者SF CyPA表达明显高于OA及Trauma患者,且与疾病活动性指标相关,提示CyPA可能在RA发病及炎症活动中起调节作用,而LPS可促进RA患者SF CyPA的表达.

Objective To observe the expression of cyclophilin A （CyPA） and the effects of lipopolysaccharide （LPS） on CyPA expression in synovial fibroblasts （SF） from patients with rheumatoid arthritis （RA） and evaluate the potential significance of CyPA in the regulation of the onset and development of inflammation process in RA patients.Methods SF were separated and cultured from synovial tissues of 12 patients with RA,9 with osteoarthritis （OA） and 5 with knee trauma.The protein and mRNA expression levels of CyPA in SF were detected by Western blot （WB） and real-time quantitative polymerase chain reaction （RT-qPCR） respectively.Correlation analysis was conducted between the protein expression of CyPA in SFs and clinical parameters.Then the effects of LPS on CyPA in SF from 3 groups were detected.Results The expression levels of CyPA protein and mRNA in RA group were 0.86 ± 0.47 and 0.54 ±0.22 respectively,significantly higher than those in OA group （0.40 ±0.31 and O.03 ±0.02,P 〈 0.05） and trauma group （0.34 ±0.21 and 0.03 ±0.01,P 〈0.05）.The protein expression level of CyPA in SF of RA group had positive correlations with erythroeyte sedimentation rate （ESR）,rheumatoid factor （RF） and swelling joint counts （SJC） （P 〈 0.05）.After LPS treatment,CyPA protein and mRNA levels were 2.65 ± 1.16 and 1.82 ±0.39 in RA SF and they were significantly higher than those in RA SF without LPS treatment （P 〈 0.05）.The CyPA expression of SF from OA and trauma groups slightly decreased after LPS treatment.However the differences were not statistically significant （P 〉0.05）.Conclusion The expression of CyPA is up-regulated in SF and it is positively correlated with ESR,RF and SJC in RA patients.It indicates that CyPA may be involved in the regulation of the onset and development of inflammation process of RA.And LPS may promote the expression of CyPA in SF of RA patients.