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老年创伤患者外周血髓源抑制细胞的变化及其意义

Variation and significance of CD14^-/CD11b^+/CD33^+myeloid derived suppressor cells in elderly patients with multiple injuries
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摘要 目的探讨老年创伤患者外周血中髓源抑制细胞(MDSCs)比例变化及其临床意义。方法收集26例青年创伤患者(18~30岁)和21例老年创伤患者(>60岁),以CD14-/CD11b+/CD33+作为MDSCs标志物,流式细胞术(FCM)检测MDSCs比例,酶联免疫吸附试验(ELISA)检测血清白介素-10、转化生长因子-β和C反应蛋白水平,分析MDSCs比例的变化情况及其临床意义。结果伤后1d,老年组和青年组创伤患者外周血MDSCs比例无显著差异(P>0.05)。伤后3d和7d,老年组创伤患者外周血MDSCs比例均高于青年组,差异有显著意义(P<0.01)。MDSCs比例和血清转化生长因子-β、白介素-10和C反应蛋白水平均有相关性。结论老年创伤患者外周血MDSCs比例较青年组显著增高,可能是老年创伤患者更容易发生免疫抑制的重要机制。 Objective To explore the variation and significance of CD 14 -/CD11b＋/CD33 ＋myeloid de-rived suppressor cells （ MDSCs ） in elderly patients with multiple injuries . Methods Totally 26 young patients （ age 18-30 years ） and 21 elderly patients （ age 〉60 years ） with multiple injury were enrolled in this study . CD14 -/CD11b＋/CD33 ＋MDSCs in peripheral blood were determined by flow cytometry （ FCM） at 1,3 and 7days after trauma.Serum transforming growth factor-β（TGF-β）,interleukin-10 and C-reactive protein were determined by ELISA.Results The percentages of CD14 -/CD11b＋/CD33 ＋MDSCs in peripheral blood of elderly patients at 3 and 7days after trauma were significantly higher than those of young patients （P0.05）.The percentages of CD14 -/CD11b＋/CD33 ＋MDSCs in peripheral blood of elderly patients with multiple injury were closely correlated with serum TGF -β,interleukin-10 and C-reactive protein level （P 〈0.05）.Conclusion The percentages of CD14 -/CD11b＋/CD33 ＋MDSCs in peripheral blood were significantly increased in elderly patients with multiple injuries .Our results indicated that accumulation of CD 14 -/CD11b＋/CD33 ＋MDSCs may be an important mechanism of aging-related immune dysfunction in elderly patients with multiple injuries .
作者 薛晨晨 何方军 赵建立 尹燕华 白祥军 李占飞
机构地区 华中科技大学同济医学院附属同济医院创伤外科 宜昌市夷陵区人民医院急诊科 华中科技大学同济医学院生物化学与分子生物学系
出处 《创伤外科杂志》 2014年第3期215-218,共4页 Journal of Traumatic Surgery
基金 武汉市科技计划(201161038339) 国家十二五支撑计划(2012BAI11B00) 湖北省医学领军人才培养工程专项经费资助
关键词 创伤 细胞 感染 免疫 trauma cell infection immune
分类号 R392.3 [医药卫生—免疫学]
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参考文献12

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二级参考文献9

  • 1Annane D,Bellissant E,Cavaillon JM.Septic shock.Lancet,2005,365(9453):63-78.
  • 2Gabrilovich DI,Nagaraj S.Myeloid-derived suppressor cells as regulators of the immune system.Nat Rev Immunol,2009,9(3):162-74.
  • 3Ostrand-Rosenberg S,Sinha P.Myeloid-derived suppressor cells:linking inflammation and cancer.J Immunol,2009,182(8):4499-506.
  • 4Nagaraj S,Gabrilovich DI.Tumor escape mechanism governed by myeloid-derived suppressor cells.Cancer Res,2008,68 (8):2561-2563.
  • 5Makarenkova VP,Bansal V,Matta BM,et al.CD11b+/Gr-1+myeloid suppressor cells cause T cell dysfunction after traumatic stress.J Immunol,2006,176(4):2085-2094.
  • 6Delano M J,Scumpia PO,Weinstein JS,et al.MyD88-dependent expansion of an immature GR-1 + CD11 b + population induces T cell suppression and Th2 polarization in sepsis.J Exp Med,2007,204(6):1463-1474.
  • 7Almand B,Clark JI,Nikitina E,et al.Incrcased production of immature myeloid cells in cancer patients.A mechanism of immunosuppression in caneer.J Immunol,2001,166(1):678-689.
  • 8Nonaka K,Saio M,Suwa T,et al.Skewing the Th cell phenotype toward Th1 alters the maturation of tumor-infiltrating mononuclear phagocytes.J Leukoc Biol,2008,84(3):679-688.
  • 9Bryk JA,Popovic PJ,Zenati MS,et al.Nature of myeloid cells expressing arginase 1 in peripheral blood after trauma.J Trauma,2010,68(4):843-852.
创伤外科杂志
2014年 第3期

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