阻塞性睡眠呼吸暂停低通气综合征合并高血压动物模型的建立

Animal model of obstructive sleep apnea hypopnea syndrom with hypertension in rats

目的探讨一种建立阻塞性睡眠呼吸暂停低通气综合征(OSAHS)合并高血压动物模型的新方法。方法将普通级雄性Wistar大鼠(8周龄)20只,适应性喂养1周,随机分为2个组(每组10只),一组为对照组不做处理,另一组为实验组——咽腔阻塞组,应用医用透明质酸钠凝胶(1 mL)咽腔两侧及舌根部注射,造成咽腔狭窄模型。两组均普食喂养。浅麻醉状态下对两组大鼠进行口鼻气流、血氧饱和度等睡眠呼吸监测并阶段性测量动脉收缩压判断是否形成OSAHS及高血压。结果①在浅麻醉状态下,实验组造模前后大鼠呼吸暂停指数(apnea index,AI)、最长呼吸暂停时间及最低血氧饱和浓度之间差异均有统计学意义(P<0.05);②实验组和对照组大鼠在造模前动脉收缩压(SBP)的差异没有统计学意义,随着时间延长实验组动脉收缩压逐渐升高,8周时实验组与对照组比较动脉收缩压差异有统计学意义(P<0.01)。结论咽部注射透明质酸法是一种稳定、可靠的建立大鼠OSAHS合并高血压模型的方法。

Objective To build an animal model of obstructive sleep apnea hypopnea syndrom （OSAHS） with hyperten sion. Methods 10 Wistar rats which were injected 1 mL Sodium Hyaluronate into the palatoglossal arch, palatopharyn geal arch and root of the tongue of served as the experimental group, and the other 10 Wistar rats as the control group. The electroencephalogram and oro-nasal airflow were recorded by PSG when rats were under anesthesia and measuring arterial systolic pressure （SBP） and OSAHS periodic. Results ① The experimental group showed differences in apnea index （ AI）, the longest apnea and the lowest concentration of blood oxygen saturation before and after injection of So dium Hyaluronate when under light anesthesia （ P 〈 0.05 ） ; ② SBP in the two groups were insignificant before building the model. The SBP of the experimental group began to rise at 4 weeks and were obvious increased when compared with the control group at 8 weeks （P 〈 0.01 ）. Conclusion The animal model of OSAHS with hypertension in rats estab lished by injection of Sodium Hyaluronate was steady and credible.