大叶蚁塔组织培养技术

Tissue Culture of Gunnera manlcata L.

以大叶蚁塔茎尖作为外植体诱导出无菌苗,再以无菌苗的嫩叶诱导不定芽发生,研究了其离体培养和不定芽再生的过程,成功建立了低成本的快速繁殖技术体系。结果表明:(1)无菌苗增殖培养基为MS+BA 1.0mg·L-1+NAA 0.1 mg·L-1,培养30 d,增殖率稳定为3.80;(2)叶片可直接诱导再生出不定芽,其最佳培养基为MS+ZT 1.0 mg·L-1+NAA 0.1 mg·L-1,诱导率达95%,分化幼苗众多;(3)生根培养基为1/2MS+NAA1 mg·L-1+蔗糖20 g·L-1时,生根率达95%。

Using the apical buds of Gunnera manlcata as explants to induce the aseptic the new leaves of aseptic seedling to induce the adventitious buds, apical buds of G. manlcata were studied in this paper, and the seedlings and then using in vitro culture and regeneration process of quick propagation system in low cost was successfully established. The result showed： （ 1 ） Multiplication stage ： MS ＋ BA 1.0 mg · L - 1 ＋ NAA 0. 1 mg · L- -, cultured for 30 d, the stable multiplication rate was 3.80; （2） Induction stage： MS ＋ ZT 1.0 mg · L-1 ＋ NAA 0.1 mg · L-1, which was proved to be superior to the others to be used to induce apical buds directly from the leaf explants, the induction rate reached 95% ; （3） Rooting stage： 1/2MS ＋ NAA 1 mg · L-1 ＋ sugar 20 g · L-1 , the rooting rate reached 95%.