山新杨组培苗生根移栽方法

Rooting and Transplanting Method of Populus davidiana × P.bolleana Tissue Culture Seedlings

从炼苗，保湿，防污染等方面，研究出一种新型高效的山新杨组培苗生根移栽方法。首先，获取山新杨组培苗，将其用液体生根培养基在大试管中生根12～15d；再放入无菌自来水中水培炼苗2d；之后将幼苗移栽入土，上扣透明塑料水杯保湿，15d后移去水杯；带土移栽到大田中。此法可使组培苗移栽成活率提高至98％以上，且本法经济简便，规模小，效率高，也适用于其它木本植株，便于推广。

A new typical and high eificient rooting and transplanting method ot Poputus davidana x r. Ootteana was set up from hardening off seedlings, moisturizing and pollution prevention in the study. Firstly, put the tissue culture P. davidiana x P. bolleana seedlings in large test tubes with liquid medium for rooting for 12 - 15 d. Secondly, refine hydroponic seedlings in the aseptic water for 2 d. Then, transplant the seedlings into soil and cover with plastic cups to moisturizing, which will be removed after 15 d. At last, the 17 d old seedlings with soil were transplanted into the field. Using the above method, the survival rate of transplanting seedlings can increase to more than 98%. The advantage of the method is not only economic, simple, small-scaled and high efficient but also suitable for many other woody plants, so it is easy to generalize.