吉林12种蒲公英HPLC指纹图谱研究

HPLC Fingerprints of 12 Taraxacum from Jilin Provinc

通过比较吉林产不同种蒲公英HPLC指纹图谱,探讨吉林产蒲公英HPLC指纹图谱的特点,为蒲公英药材的质量控制提供理论参考。采用HPLC方法,以Agilent Extend-C18(250 mm×4.6 mm,5μm)为色谱柱,以甲醇-0.5%冰醋酸水溶液梯度洗脱;检测波长为323 nm,流速为1.000 mL·min-1,柱温35℃,进样量为20μL,检测了12种吉林产不同种类蒲公英药材,确立了9个共有峰,建立了蒲公英对照指纹图谱,计算各被测样品的HPLC指纹图谱的整体相似度,并指认了绿原酸、咖啡酸、总黄酮3个特征峰,比较了上述成分在不同药材中的含量,定量结果表明,3种成分平均含量分别为0.027%、0.026%、0.128%。所建立的指纹图谱具有良好的精密度、重现性和稳定性,可作为吉林产蒲公英药材的质量控制标准。

To explore the characteristics of HPLC fingerprints of Taraxacum in Jilin province and provide a theoretical reference for the quality control of dandelion herbs, the HPLC fingerprints of Taraxacum in Jilin province were compared. HPLC analysis was carried out in Agilent Extend-Cls （4.6 mm x 250 mm, 5 μm） column with a mobile phase of methanol and 0.5% acetic acid water at the flow of 1. 000 mL/min, the detection wavelength was 323 nm, the temperature was 35℃, the injection volume was 20 μL. The established fingerprint of herba Taraxaci was used to determine 12 batches of samples from different sources. Nine peaks were identified as the character fingerprints through similarity analysis and 3 compounds among them were determined as chlorogenic acid, caffeic acid and flavonoids by HPLC. Quantitative results showed that the average contents of three kinds of ingredients were 0.027%, 0. 026% and 0. 128%, respectively. The method has good precision, reproducibility and stability, and can be used as the quality control of Herba taraxaci in Jilin Province.