氨培养胎鼠神经细胞钙离子浓度变化与凋亡的相关性

The Correlation between Concentr ation of Intracytoplasm Calcium Ion and Apoptosis in Fetal Rat Neuron Induced by Ammonia in vitro

目的观察体外培养神经细胞在氨作用下钙离子浓度变化与凋亡的关系,探讨氨毒性机制.方法体外培养胎鼠神经细胞7 d,实验分组为对照组、低氨组和高氨组;分别作用24 h后,用流式细胞仪测定神经细胞凋亡比率,用Fluo-3/AM荧光探针测定细胞内钙离子浓度.结果对照组神经细胞有少量凋亡,细胞内钙离子浓度正常,随着NH4CL浓度的提高,细胞内钙离子浓度明显升高,神经细胞凋亡增加.低氨组和对照组相比差异有统计学意义(P<0.05),高氨组和低氨组比较,差异有统计学意义(P<0.05).结论氨诱导神经细胞凋亡与细胞内钙离子浓度相关.

Objective To investigate the correlation between intracellular free calcium ion concentration and apoptosis in neurons induced by ammonia in Vitro,to explore the mechanism of ammonia intoxication.Methods Fetal rat neurons were cultured in vitro for 7 days,Neurons were assigned to the control group,low concentration of ammonia group,and high concentration of ammonia group.After treated for 24 h,Flow cytometry was used to count apoptosis cells,Flluo-3/Am was used to detect the variation of calcium in neurons.Results There was significant difference between control group and the low concentration of ammonia group.The difference was statistically significant between the low concentration of ammonia group and high concentration of ammonia group.Conclusion Ammonia might induce apoptosis of fetal rat neuron in vitro; there is striking correlation between intracellular free calcium ion concentration and neuronal apoptosis induced by ammonia.