骨癌痛大鼠髓内SIGIRR的表达变化

The expression of SIGIRR in rats with bone cancer pain

目的本研究针对目前日益增长的骨癌痛病患的疼痛问题进行试验探讨。建立癌痛动物模型寻找有效缓解疼痛的方法,进一步探讨骨癌痛大鼠模型中髓内细胞蛋白SIGIRR的表达水平的变化。方法建立SD大鼠胫骨癌痛模型,随机分为空白组(10只)和骨癌痛模型组(10只)。通过大鼠痛行为学测试、影像学、组织学方法对大鼠胫骨癌痛的模型进行验证。运用RT-PCR和Western blot检测髓内蛋白细胞LTR4和SIGIRR表达变化。结果 Walker256大鼠乳腺癌细胞制备大鼠胫骨癌痛的模型骨癌造模成功。模型组的体质量不增或者降低(P<0.01)、SAPS显著增加(P<0.01)、PWTL差异不大(P>0.05)。RT-PCR检测的TLR4结果显示,骨癌痛模型组比空白组表达明显增加。Western blot检测骨癌痛模型组比空白组髓内蛋白细胞SIGIRR明显下降。结论骨癌痛大鼠髓内蛋白细胞SIGIRR表达的降低可能与骨癌痛的发生机制相关。

This study designed to explore the changes of single Ig IL-1R-related molecule （SIGIRR） expression in the spinal dorsal horn of rats with bone cancer pain. Firstly, adult male Sprague-Dawley rats were randomly divided into two groups： bone cancer pain group （n=10） and control group （n=10）. Then, series of indexes were observed including general condition, weight bearing ability, ambulatory pain together with bone radiology and bone histology. Furthermore, Western blot and RT-PCR were used to observe the changes of SIGIRR and TLR4 in medulla spinalis. Results indicated that rat model of hyperalgesia and allodynia was achieved after intra-tibial injection of Walker 256 cells. The weight change and ambulatory score （SAPS） of control rats and bone caner rats were significant different （P〈 0.01）. However difference of paw withdrawal latency （PWTL） between control rats and bone cancer rats was not remarkable （P 〉 0.05）. Furthermore, TLR4 protein in bone cancer pain group was highly increased compared with the control group; Western blot revealed that SIGIRR protein in bone cancer pain group was evidently reduced, as compared with control group. In conclusion, the changes of SIGIRR expression may involve in the development of bone cancer, and this finding might provide a new analgesic strategy for reducing cancer pain.