间充质干细胞移植对正己烷致周围神经病大鼠神经电生理的影响

Effects of mesenchymal stem cells transplantation on neurophysiological of peripheral neuropathy rats induced by n-Hexan

目的观察大鼠骨髓间充质干细胞(mesenchymal stem cells,MSCs)移植对正己烷致周围神经病大鼠神经电生理的影响。方法 SD大鼠54只随机分为对照组、低剂量染毒组和高剂量染毒组,低剂量组和高剂量组均用2,5-己二酮(2,5-HD),分别以200 mg/kg和400 mg/kg的剂量进行腹腔注射染毒,对照组用生理盐水进行腹腔注射。染毒6周后,以上各组均随机分为2组,分别任取1组大鼠尾静脉移植MSCs,每只大鼠移植1×106个MSCs。染毒及MSCs移植过程中,观察大鼠的一般状态和步态变化,并用肌电图与诱发电位仪记录大鼠尾神经传导潜伏期和神经传导速度。结果大鼠染毒6周时,低、高剂量组的步态评分分别升高至2.87±0.28和3.78±0.24、神经传导潜伏期分别延长为(5.15±0.29)ms和(6.75±0.35)ms、神经传导速度分别减低为(13.20±0.51)m/s和(8.00±0.43)m/s。MSCs移植后,随移植时间的延长,模型大鼠步态评分下降,神经传导潜伏期和神经传导速度逐渐恢复。移植5周后,低、高剂量组的神经传导潜伏期分别恢复21.63%和16.08%,神经传导速度分别提高13.38%和38.71%,与未移植MSCs的对照组相比,差异有显著性意义(P<0.05)。结论 MSCs移植能明显改善正己烷致周围神经病大鼠的行为学和神经电生理。

Objective To evaluate the effects of mesenchymal stem cells transplantation on neurophysiology of rats with peripheral neuropathy induced by n -Hexan.Methods Total 54 rats were randomly divided into 3 groups, of which the rats were given at 0mg/kg, 200 mg/kg and 400 mg/kg 2,5-HD i.p.After 6 weeks, the rats injected with 2,5-HD were divided into two groups , and the rats in one group were transplanted with MSCs at 1 ×106 .The gait, distal latency and motor nerve conduction velocity were observed .Results At 6 weeks, the gait score was 3.78 ±0.24, the distal latency was （6.75 ±0.35）ms and motor nerve conduction velocity was （8.00 ±0.43）m/s in the rats of 400 mg/kg group.And these data in 200 mg/kg group were 2.87 ±0.28, 5.15 ±0.29 and 13.20 ±0.51 respectively.After MSCs transplantation, the recovered gait , decreased distal latency and increased motor nerve conduction velocity were observed in transplantation groups.At 5 weeks after MSCs transplantation , the gait score significantly decreased to 28.63%, the distal latency was recovered to 21.63%and 16.08%, and the increased motor nerve conduction velocities were 13.38%and 38.71%in 200 mg/kg group and 400 mg/kg group, respectively.Conclusion MSCs transplantation can improve neurobehavior and neurophysiology in peripheral neuropathy rats induced by n -Hexan.