糖耐量损害大鼠脑组织NF-κBp65及TNF-α表达水平与认知障碍的关系

Relationship between cognition disorders and impaired glucose tolerance at the level of NF-κBp65 and TNF-α in rats brain

目的研究大鼠脑组织NF-κBp65,TNF-α因子在认知障碍中的作用,从炎症角度探讨糖耐量损害对认知障碍的影响。方法高脂高糖饲养法建立糖耐量损害大鼠模型;水迷宫法检测大鼠认知功能;免疫组化法检测NF-κBp65,TNF-α表达水平。结果实验组学习记忆能力在第5周较对照组高(P<0.05),第10、15、20周逐次下降,且低于相同时间点对照组(P<0.05)。Pearson相关分析显示NF-κBp65,TNF-α阳性表达水平与大鼠认知障碍呈正相关(r=0.972,r=0.975,P<0.05)。结论糖耐量损害与大鼠认知障碍有关,炎症机制可能是其重要机制之一。

Objective To explore the function of NF-κBp65 and TNF-α of cognition disorders in rat brain, and discuss the relationship between impaired glucose tolerance and cognition disorders from the perspective of inflammatory mechanism. Methods Impaired glucose tolerance model rats were made by high fat and sugar diet;The Morris water maze was applied in the study of learning and memory in rats; Expression of NF-κBp65 and TNF-α factor in rat brain were detected using immunohistochemistry. Results Compared with contrast group, the ability of learning and memory were significantly increased （P〈0. 05） in the fifth week, while the ability in experimental group were meaningfully decreased in each model subgroup.. 10th. 15th.20th week （P〈0. 05）, and lower than the contrast group; The data were assessed by the Pearson that the expres- sion of NF-κ Bp65 and TNF- α correlated with the cognition disorders of rats. Conclusions There is a relation-ship between impaired glucose tolerance and cognition disorders, its mechanism may have some connection with the high expression of NF-κBp65, TNF-α.