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ELISA法测定动物源性生物材料中残留的α-Gal抗原

An Assay for Measuring α-Gal Epitope Expression on Xenotransplantation
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摘要 本研究用ELISA直接法分别检测新鲜心包组织、新鲜皮肤组织及新鲜骨组织中α-Gal抗原含量分别为1.076±0.2045 U/mg、3.247±0.2370 U/mg和14.53±0.3048 U/mg,组间差异有统计学意义(P<0.05)。ELISA法检测牛松质骨、经过初步处理的牛骨以及处理完成的成品骨中α-Gal抗原含量,三种组织中α-Gal抗原含量分别为14.53±0.3048 U/mg、11.62±1.065 U/mg和1.546±0.3857 U/mg,组间差异有统计学意义(P<0.05)。结果表明,ELISA直接法能够方便快捷的检测异种移植材料中α-Gal抗原含量,并且结果可信,可以为异种移植材料中抗原含量评价提供依据。 The expression of α-Gal epitope on pericardium,skin and bone were 1. 076 ± 0. 2045 U /mg,3. 247 ± 0. 2370 U /mg and 14. 53 ± 0. 3048U / mg,respectively with statistically differences between groups（ P〈0. 05）. The expression of α-Gal epitope on fresh bone,intermediate product and finished product were 14. 53 ± 0. 3048 U / mg,11. 62 ± 1. 065 U / mg and 1. 546 ± 0. 3857 U / mg, with statistically differences between groups（ P〈0. 05）. ELISA assay for measuring α-Gal epitope expression is useful for the evaluation of xenotransplantation.
作者 刘佳 盖潇潇 孙晓霞 刘成虎 傅明洁 侯丽
机构地区 山东省医疗器械产品质量检验中心 山东大学医学院
出处 《生物医学工程研究》 2014年第1期49-51,55,共4页 Journal Of Biomedical Engineering Research
基金 "十二五"国家科技支撑计划项目(2012BAI22B01) 再生型医用植入器械国家工程室PI项目(2012NELRMD007)
关键词 α-Gal抗原 ELISA 异种移植 天然抗体 排异反应 α-Gal epitope ELISA Xenotransplantation Natural antibody Reject reaction
分类号 R318 [医药卫生—生物医学工程]
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2014年 第1期

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