摘要
目的 建立中国人同一起源肿瘤不同恶性潜能前列腺癌动物模型,为研究前列腺癌转移进展机制及激素抵抗的发生机制提供理想的动物模型.方法 采用组织块外科原位移植法将人前列腺癌组织分别移植到虚拟去势组及去势组雄性裸小鼠前列腺部,成瘤后进行鼠间传代移植,选取虚拟去势组原位移植瘤、移植淋巴结转移瘤、去势组第4代淋巴结转移瘤体外培养建立细胞系,应用Boyden Chamber细胞运动实验检测细胞迁徙转移能力,描绘细胞生长曲线分析细胞增殖及激素依赖性,裸小鼠皮下异种移植瘤模型检测成瘤率、肿瘤湿重及浸润范围验证不同代移植瘤细胞在裸小鼠异种移植的生长状态.结果 第1代虚拟去势组成瘤率30%,无淋巴结转移,取移植瘤反复原位传代移植第3代成瘤率50%,盆腔淋巴结转移率40%.去势组原代移植以及来自虚拟去势组的原位移植瘤反复原位移植均未见移植瘤,源自虚拟去势组淋巴结转移瘤组织反复原位传代移植至第4代,成瘤率33%,其中一只可见盆腔淋巴结转移.三种细胞系细胞生长、迁徙以及体外裸小鼠成瘤率均有显著差别(P<0.05).结论 裸小鼠前列腺癌原位移植反复传代可增强肿瘤成瘤率、转移力等恶性指标.以裸小鼠淋巴结转移瘤接种去势裸小鼠可筛选出激素非依赖性异种移植瘤及转移瘤.
Objective Supplying ideal animal model for investigating the mechanism of tumor progress and hormone resistant in prostate cancer. Methods Prostate cancer tissue was obtain via prostatectomy and cut into specimens which orthotopic transplanted into castrate group and sham- castration group nude mice .T hen tumor was harvest and transplant to next generation, until emergence of lymph node metastasis in two groups.. The cell growth curve of cell lines is used to evaluate their growth condition. Boyden chamber is used to evaluate metastasis ability of cell lines. The rate of tumor formation, weight of tumor and range of tumor infiltration of three cell lines was confirmed by subcu- taneous transplant. Result The tumorgenic in sham-castration group is 30% without lymph node metastasis in first generation, after three passages is 50%, lymph node metastasis in pelvic is 40%. In castrate group, no tumor formation and lymph node metastasis in first generation. One pelvic lymph node metastasis is find after the forth passages which tumor origin from the lymph node metastasis of sham- castration group.. There are significant differences of cell proliferation transfer ability and tumor rate in vitro and vivo between three cell lines. (p〈0.05). Conclusion It's feasible that applying surgical speci- mens of prostate cancer to establishment .nude mice orthotopic graft model. It is possible to screen heterotransplant tumors and metastasis tumors without hormone dependency by graft rat's lymph node metastasis tumor into castrate nude mice.
出处
《实验动物与比较医学》
CAS
2014年第2期83-88,共6页
Laboratory Animal and Comparative Medicine
基金
浙江省医学重点学科建设计划(GJSX-010-004)
浙江省公益性技术应用研究计划项目(实验动物2011C37081)
浙江省自然科学研究基金(Y13H160104)
关键词
前列腺癌
原位异种移植
肿瘤模型
雄激素抵抗
Prostate cancer
Orthotopic xenotransplantation
Tumor model
Androgen resistance