复方甘草酸苷对溃疡性结肠炎大鼠结肠组织NF-B p65及COX-2表达的影响

Effect of Compound Glycyrrhizin on Expressions of Colonic NF-κB p65 and COX-2 in Ulcerative Colitis Rats

目的 观察复方甘草酸苷对溃疡性结肠炎大鼠核因子-κB p65（NF-κB p65）及环氧合酶2（COX-2）的影响并探讨其作用机制.方法 成年SD大鼠40只,随机分成4组,即正常对照组、模型组、复方甘草酸苷治疗组及柳氮磺胺吡啶（SASP）治疗组.正常对照组不做任何处理,其余3组用2.5％三硝基苯磺酸钠/乙醇溶液灌肠制备溃疡性结肠炎模型,模型组用生理盐水灌肠3d后处死,复方甘草酸苷治疗组每日用复方甘草酸苷溶液（40 mg/kg）连续灌肠7d后处死;SASP治疗组每日用SASP溶液（0.5 g/kg）连续灌肠7d后处死.经不同处理和治疗后,运用免疫组化染色（SABC法）检测NF-κB p65及COX-2表达.结果 与正常对照组相比,模型组、复方甘草酸苷治疗组及SASP治疗组的NF-κB p65表达水平明显上升（P＜0.01）; COX-2的表达水平亦明显上升（P＜0.01）;与模型组相比,复方甘草酸苷组及SASP治疗组的NF-κB p65、COX-2的表达水平均明显下降（P＜0.01）.结论 复方甘草酸苷对大鼠溃疡性结肠炎有良好的治疗作用,其作用机制可能与降低大鼠结肠组织中NF-κB p65及COX-2的表达有关.

Objective To investigate the experimental therapeutic effect of the Compound Glycyr- rhizin on the expression of NF-κB p65 and COX-2 in rats with ulcerative eolitis（UC） induced by trinitrobenzene sulfonic acid/ethanol （TNBS/ethanol）. Methods Forty adult Sprague-Dawley rats were randomly divided into 4 groups（n=10）, Normal control group received no treatment, while the rest groups used trinitrobenzene sulfonic acid/ethanol enema to generate ulcerative colitis models. UC model group received saline water enema and decapitated three days later, Compound glycyrrhizin glycosides treatment group daily received glycyrrhizin glycosides solution enema, 40 mg · kg-1 · d1, Continuous enema 7 days and decapitated,, Sulfasalazine （SASP） treatment group daily received SASP solution enema, 0.5 g · kg-1 · d% Continuous enema 7 days and decapitated, Each group received different treatment as described above, NF-κB p65 and COX-2 were detected by immunohistochemistry. Results Compared with Normal control group, the level of NF-κB p65 was significantly increased in UC model group, SASP treatment group and Compound glycyrrhizin glycosides treatment group [0.25 ±0.11 vs 5.58±0.85 vs 3.36 ± 0.66 vs 3.21 ± 0.58, P〈0.01], and the level of COX-2 was significantly increased [0.21 4± 0.13 vs 5.54 ± 0.40 vs 3.23 ± 1.05 vs 3.16 ±0.94, P〈0.01]. Compared with Model group, the level of NF-eB p65 was significantly decreased in SASP treatment group and Compound glycyrrhizin glycosides treatment group [5.58 ± 0.85 vs 3.36 ±0.66 vs 3.21 ± 0.58, P〈0.01], and the level of COX-2 was significantly decreased [5.54 ± 0.40 vs 3.23 ± 1.05 vs 3.16 ± 0.94, P〈0,01]. Conclusion Compound Glyeyrrhizin has therapeutic effect on ulcerative colitis in rat. The mechanism is associated with the reduced levels of NF-κB p65 and COX-2.