ZDF（fa/fa）大鼠的糖脂代谢特点与胰岛素抵抗特性

The Characteristics of Glucolipid Metabolism and Insulin Resistance in ZDF（fa/fa） Rats

目的 观察ZDF（fa/fa）大鼠高脂饲喂后的糖脂代谢特点和胰岛素抵抗特性.方法 取ZDF（fa/fa）大鼠6只（ZDF模型组）,每日饲喂高脂饲料,另取ZDF（fa/＋）大鼠6只（ZDF对照组）,饲喂普通饲料,连续饲喂6周;每周称重,隔周检测GLU、TG、CHOL、TC、HDL-C、LDL-C,计算TC/HDL-C和AI,于第6周检测空腹胰岛素,并进行糖耐量与胰岛素耐量试验,计算HOMA-IR和HOMA-β.结果 ZDF（fa/fa）大鼠高脂饲喂后空腹血糖、血脂水平迅速升高（p＜0.01）,TC/HDL-C和AI显著升高（P＜0.01）,饲喂第4周后趋于稳定;第6周的空腹胰岛素和HOMA-IR显著升高（P＜0.01）,而HOMA-β则显著降低（P＜0.01）.结论 ZDF（fa/fa）大鼠饲喂高脂饲料后出现高血糖伴高脂血症、糖耐量异常和高胰岛素血症,同时出现胰岛素耐量增加和胰岛素敏感性降低,较适合于胰岛素抵抗引起的2型糖尿病和代谢综合症的研究.

Objective To observe the characteristics of glucolipid metabolism and insulin resistance in ZDF rats after feeding with high fat diet. Methods Six ZDF （fa/fa） rats in model group were fed with high fat diet, 6 ZDF （fa/＋） rats in control group, fed with normal diet, lasted for 6 weeks. The body weight was measured every week. Glucose, cholesterol, triglycerides, high-density lipoprotein, low density lipoprotein were measured every two weeks, TC/HDL-C and AI were calculated. In 6th week, the fasting insulin, OGTT and ITT were measured. HOMA-IR, HOMA-β were calculated. Results After feeding with high fat diet, the blood glucose and lipid levels in ZDF model group were rapidly increased （P〈0.01）, TC/HDL-C and AI were significantly increased （P〈0.01）, after 4 weeks, they remained stable.In 6th week, fasting insulin and HOMA-IR were significantly increased （P〈0.01）, but HOMA-β was signifi- cantly decreased （P〈0.01）. Conclusion ZDF rats induced by high fat diet, exhibits hyperglycemia with hyperlipidemia, abnormal glucose tolerance and hyperinsulinemia, and also exhibits increased insulin tolerance and decreased insulin sensitivity. ZDF rats are suitable to study tyjpe 2 diabetes mellitus and metabolic syndrome which caused by insulin resistance.