硫化氢对人结肠癌细胞增殖和迁移的影响

Effect of hydrogen sulfide on human colon cancer SW480 cell proliferation and migration in vitro

目的研究H2S对人结肠癌SW480细胞增殖和迁移的作用,及其调控的分子机制。方法用不同浓度H2S处理结肠癌SW480后,采用MTT法检测结肠癌SW480细胞增殖能力的改变,细胞划痕实验观察SW480细胞迁移能力的改变,Western blot实验检测MMP-2、MMP-9和SIRT1表达水平的改变。结果 MTT实验,24、48、72 h 3个时间组50、100、200、400μmol/L H2S处理后,与相应时间对照组相比,H2S均能促进细胞的增值;细胞迁移实验结果显示NaHS(100μmol/l)处理组迁移能力增强且明显高于正常对照组(P<0.01),Western blot结果显示NaHS(100μmol/l)处理组MMP-2和MMP-9的表达水平增强且明显高于正常对照组(P<0.05,0.01),且NaHS(100μmol/l)处理组SIRT1表达水平增强。结论 H2S能够促进人结肠癌SW480细胞的增殖和迁移,其机制可能与上调SIRT1的表达有关。

Objective To investigate the effect of hydrogen sulfide （H2S） on the proliferation and migration of human colon cancer SW480 cells and explore its molecular mechanisms. Methods The proliferation of SW480 cells exposed to different concentrations of NaHS for varying time lengths was analyzed by MTT assay, and the changes in cell migration was evaluated using wound-healing assay. The changes in the expression levels of MMP-2, MMP-9 and SIRT1 protein were detected by Western blotting in the exposed cells. Results Compared with the control cells, SW480 cells exposed to 50, 100, 200, or 400μmol/L NaHS for 24, 48 and 72 h all showed increased proliferative activity. NaHS treatment at 100 μmol/L significantly promoted the cell migration （P＆lt;0.01） and enhanced the cellular expressions of MMP-2 （P＆lt;0.05） and MMP-9 （P＆lt;0.01） proteins;NaHS exposure （100μmol/L） also resulted in up-regulation of SIRT1 expression in SW480 cells. Conclusion H2S can promote proliferation and migration of SW480 cells in vitro, the mechanism of which may involve up-regulated expression of SIRT1.