超高效液相色谱-质谱联用技术分析新生牛和胎牛血清组分

Ultra-high performance liquid chromatography-mass spectrometry for analysis of newborn and fetal bovine serum components

目的探讨超高效液相色谱-质谱联用(UPLC/MS)技术对新生牛和胎牛血清组分中活性成分的分析和鉴别。方法采用UPLC/MS技术分析Gibco公司新生牛血清和Hyclone公司胎牛血清组分中的活性成分。结果特征图谱分析新发现:胎牛血清组分种类多于新生牛血清中组分,其质荷比(m/z)分别为498、273和448的物质。结论 UPLC/MS技术可用于胎牛血清及新生牛血清的区分和鉴别,检测出胎牛血清中的几种成分可能是促进细胞发生增殖分化的主要活性物质。

We used ultra-high performance liquid chromatography-mass spectrometry （UPLC/MS） for analyzing and identifying the active components of newborn calf serum （NCS） and fetal bovine serum （FBS）. The results demonstrated significant differences in the components between NCS and FBS. FBS appeared to have more complex components than NCS, with mass to ratios （m/z） of the substances of 498, 273 and 448. These substances in FBS may be the main active components to support the proliferation and differentiation of cells.