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衣原体噬菌体衣壳蛋白Vp1及其高保守区二级结构及B细胞表位研究 被引量：2

Bioinformation study of capsid protein Vpl high conservative region parts from Chlamydiaphage
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摘要 目的:分析衣原体噬菌体Vp1蛋白及其高保守区的二级结构并预测其B细胞表位。方法:以ClustalX程序比对各株衣原体噬菌体衣壳蛋白Vp1序列获得高保守区序列。以Vp1氨基酸序列为基础,采用Gamier-Robson法、Chou-Fasman法、Eisenberg法和Karplus-Schulz法分析蛋白二级结构;按Kyte-Doolittle法、Emini法和Jameson-Wolf法预测蛋白的抗原表位。结果:衣原体噬菌体Vp1蛋白的二级结构以β折叠为主,有少量α螺旋;其高保守区含多个抗原位点,预测其N端1-8,103-110,158-164,189-196,322-332,427-434,478-488为优势表位。结论:衣原体噬菌体Vp1蛋白及其高保守区存在复杂的蛋白结构,可形成多个可选表位。 Objective： To predict the secondary structure and B cell epitope of capsid protein Vp 1 in high conservative region. Methods： The conservative region was acquired by comparing the Vpl protein sequences. The secondary structure of these regions were predicted by the method of Gamier-Robson, Chou-Fasman, Eisenberg and Karplus-Sehulz while its cell epitope was predicted by the method of Kyte- Doolittle ,Emini and Jameson-Wolf. Results： The main constructions of Chlamydiaphage Vpl were β regions, including several α regions. And the sections of 1-8,103-110,158-164, 189-196,322-332, 427-434,478-488 in the N-terminal of the conservative region could be the epitopes of B cell. Conclusion： The conservative region of Vp 1 protein has the complicated structures and can form into muhiple epitopes.
作者 姚卫锋 卢桂玲 谢艳秋 于旺 宋蒙蒙 李士颖
机构地区 天津市中医药研究院附属医院皮肤科 天津公安医院内三科
出处 《天津医科大学学报》 2014年第3期188-191,共4页 Journal of Tianjin Medical University
关键词 微病毒 蛋白 结构 分析 microvirus protein structure analysis
分类号 Q7 [生物学—分子生物学]
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参考文献11

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共引文献7

同被引文献18

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天津医科大学学报
2014年 第3期

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