大鼠胆管梗阻后肝脏纤维化相关细胞因子的变化

Changes in expression of hepatic fibrosis related cell factors in rats with bile duct obstruction

目的:通过结扎大鼠胆总管,模拟胆管梗阻病理状态,运用PCR技术,探讨这一病理过程相关细胞因子在胆管梗阻肝脏纤维化过程中发挥的作用.方法:实验组实施近肝门端胆总管单线结扎;对照组实施假手术,开腹游离胆总管但不结扎.术后不同时间处死大鼠,取肝组织通过PCR检测各目的基因表达情况.结果:两组间转化生长因子-β(transforming growth factor-β,TGF-β1)、COL-Ⅰ的表达差异从第1天开始有统计学意义,BDL组与对照组中HGF的表达差异从第3天开始有统计学意义.对BDL不同天数组间的表达研究发现COL-Ⅰ、TGF-β1在3-7 d无统计学意义,HGF在1-3、7-21 d无统计学意义,而其他相邻各组间均有统计学意义,COL-Ⅰ、TGF-β1指标1-3d增长趋势最明显,HGF指标3-7 d增长趋势最明显.结论:肝纤维化过程中的3-7 d是纤维化过程的特殊时期,第3天之前以促纤维化过程为主要表现;第3天开始出现抗纤维化表现,促纤维化趋势减弱,纤维化速度减慢;第7天之后为持续的肝脏纤维化进程.

AIM： To explore the roles of cytokines related to the pathological process of biliary obstruction and hepatic fibrosis. METHODS： Rats were divided into two groups： an experimental group and a control group. The experimental group underwent ligation of the common bile duct to induce bile duct obstruction （BDO）, and the control group received laparotomy only without ligation. Rats were sacrificed after the operation. Expression of genes of interest was detected by PCR. RESULTS： Expression of COL- I and transforming growth factor-β （TGF-β1） changed significantly from day 1, and that of HGF showed a significant change from day 3. In the BDL group, expression of COL- I and TGF-I showed no significant differences during the period from day 3 to day 7, and that of HGF showed no significant differences during the periods from day 1 to day 3 and from day 7 to day 21, while sta tistically significant differences were observed in other periods. These findings suggest that expression of COL- I and TGF-β1 increased fastest from days 1 to 3, and that of HGF increased fastest from days 3 to 7. CONCLUSION： The findings of the present study suggest that the period before day 3 is a stage of rapid fibrosis, the period between days 3 and 7 is a stage of slow fibrosis, and the period after day 7 is a stage of continuous progression of fibrosis.