矢车菊素-3-葡萄糖苷对肥胖大鼠体重、血脂及胰岛素抵抗的影响

Cyanidin-3-glucoside attenuates body weight gain, serum lipid concentrations and insulin resistance in high-fat diet-induced obese rats

目的矢车菊素-3-葡萄糖苷(C3G)是花青素的主要活性成分。该研究观察C3G对肥胖大鼠体重、血脂及胰岛素抵抗的影响。方法 3周龄雄性Sprague-Dawley大鼠30只按随机数字表法随机分为对照组(n=8)和高脂饮食组(n=22),分别以普通饲料及高脂饲料喂养。5周后再将高脂饮食诱导的17只肥胖大鼠随机分为肥胖组(n=8)和C3G组(n=9)。C3G组以C3G 100 mg/(kg·d)灌胃,其余两组以等量生理盐水灌胃。连续灌胃5周后准确称量并记录大鼠体重、内脏脂肪量及进食量,全自动生化分析仪测定空腹血糖(FPG)、总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C),放免法测血清胰岛素(Fins),ELISA测血清脂联素,并计算胰岛素抵抗指数(IRI)、动脉硬化指数(AI)和食物平均能量利用率(FER)。结果 C3G组大鼠体重、内脏脂肪量、平均FER、TC、TG、LDL-C、AI、FPG、Fins及IRI明显低于肥胖组(P<0.05),而HDL-C及血清脂联素明显高于肥胖组(P<0.05)。结论 C3G可减缓肥胖大鼠体重及内脏脂肪量增加,改善肥胖相关糖脂代谢紊乱及胰岛素抵抗,增加血清脂联素分泌是其可能机制之一。

Objective Cyanidin-3-glucoside （C3G） is the main active ingredient of anthocyanidin. This study aimed to evaluate the effects of C3G on body weight gain, visceral adiposity, lipid profiles and insulin resistance in high- fat diet-induced obese rats. Methods Thirty male Sprague-Dawley rats were randomly divided into a control group （n=8） and a high fat diet group （n=22）, and were fed with standard diet or high fat diet. Five weeks later, 17 high-fat diet-induced obese rats were randomly given C3G [100 mg/（kg·d）] or normal saline via intragastric administration for 5 weeks. Five weeks later, body weight, visceral adiposity and food intake were measured. Blood samples were collected for detecting fasting glucose, serum insulin, lipid profiles and adiponectin. Insulin resistance index, atherosclerosis index and average feed efficiency ratio were calculated. Results C3G supplementation markedly decreased body weight, visceral adiposity, average feed efficiency ratio, triglyceride, total cholesterol, low density lipoprotein cholesterol, fasting glucose, serum insulin, insulin resistance index and atherosclerosis index in high-fat diet-induced obese rats. C3G supplementation normalized serum adiponectin and high density lipoprotein cholesterol levels in high-fat diet- induced obese rats. Conclusions Cyanidin-3-glucoside can reduce body weight gain, and attenuate obesity-associated dyslipidemia and insulin resistance in high-fat diet-fed rats via up-regulating serum adiponectin level.