MIF、IL-17、IL-10在肝细胞癌组织中的表达及临床意义

Expression of MIF,IL-17 and IL-10 in hepatocellular carcinoma and their clinical significance

目的检测肝细胞癌(HCC)组织中巨噬细胞移动抑制因子(MIF)、白细胞介素(IL)-17和IL-10的表达,并探讨其临床意义。方法收集2010年9月至2013年3月52例术后HCC组织及其周围肝硬化组织和20例正常肝脏组织,采用免疫组化染色法检测各组织中MIF、IL-17和IL-10蛋白的表达,并分析其表达与HCC临床病理特征的关系。结果 52例HCC组织中MIF、IL-17和IL-10蛋白的阳性表达率分别为78.8%、76.9%、28.8%,相应的癌旁肝硬化组织中分别为75.0%、73.0%、32.7%,20例正常肝组织中分别为20.0%、25.0%、70.0%。HCC和肝硬化组织中MIF和IL-17的阳性表达率高于正常肝组织,而IL-10的阳性表达率则低于正常肝组织,差异均有统计学意义(P<0.05)。HCC组织中MIF、IL-17、IL-10表达与肿瘤直径、分化程度相关(P<0.05),而与年龄、性别、癌栓形成及Child-Pugh分级均无关;HCC组织不同MIF、IL-17、IL-10表达与1年生存率相关。结论 MIF、IL-17及IL-10的表达与HCC发生、发展及预后密切相关。

Objective To detect the expression of macrophage migration inhibitory factor（ MIF） , interleukin（ IL）-17 and IL-10 in hepatocellular carcinoma（ HCC） , and discuss their clinical significance. Methods Fifty-two cases of HCC tissues, the adjacent liver cirrhosis（ LC） tissues of HCC and 20 cases normal liver samples in the department of hepatobiliary surgery, Lanzhou General Hos-pital, Lanzhou Command, from September 2010 to March 2013 were collected. Immunohistochemistry technique was used to detect the expression of MIF, IL-17 and IL-10 in tissues of three groups. The relationship between the expression and clinicopathological features of HCC, such as the tumor size, degrees of differentiation and other features were analyzed. Results The positive rates of MIF, IL-17and IL-10 were 78？8%,76？9%,28？8% in HCC tissues respectively;75？0%,73？0%,32？7% in LC tissues;and 20？0%,25？0%, 70？0% in the normal liver tissues. Compared with the normal liver tissue, the MIF and IL-17 expression of HCC and LC tissues were higher, and IL-10 expression was lower, with statistical significances （ P＆lt;0？05） . The expression of MIF, IL-17 and IL-10 in HCC tis-sues was related to the tumor size, degrees of differentiation, not related to age, gender, tumor thrombus and Child-Pugh stage. The different expression of MIF, IL-17, IL-10 in HCC tissues was related to one year survival rate of HCC patients. Conclusion The ex-pression of MIF, IL-17 and IL-10 are closely correlated with the occurence, development and prognosis of HCC.