两种抗凝剂对实验用小型猪血液生理指标的影响

Impact of two anticoagulants EDTA-3K and heparin lithium on blood physiological parameters of miniature pigs

目的比较分析两种抗凝剂对实验用小型猪血液生理指标的影响。方法将22只成年的实验用小型猪,分别用EDTA三钾和肝素锂抗凝采血后,用日本光电MEK-7222K血球分析仪测定血液生理指标。结果使用不同的抗凝剂,同一批样本的血液生理指标中单核细胞绝对值(MON)有差异(P<0.05),平均红细胞体积(MCV)、平均红细胞血红蛋白浓度(MCHC)、血小板比积(PLT)、血小板平均体积(PCT)、血小板平均体积(MPV)、淋巴细胞绝对值(LYM)、嗜酸性粒细胞绝对值(EOS)、嗜碱性粒细胞绝对值(BAS)、淋巴细胞绝对值(LYM)%、单核细胞绝对值(MON)%、嗜酸性粒细胞百分率(EOS)%和嗜碱性粒细胞百分率(BAS)%这12个指标有显著差异(P<0.01);EDTA三钾抗凝的血液生理指标中血小板比积(PLT)、中性粒细胞绝对值(NEUT)和中性粒细胞百分率(NEUT)%有性别差异(P<0.05),MON、EOS、MON%和EOS%性别差异有显著性(P<0.01);肝素锂抗凝的血液生理指标中血小板体积分布宽度(PDW)、中性粒细胞绝对值(NEUT)和LYM%有性别差异(P<0.05),MON、EOS、MON%、NEUT%和EOS%性别差异有显著性(P<0.01)。结论不同的抗凝剂对实验用小型猪血小板和白细胞分类计数参数影响显著,进行相关实验时应妥善选择血液抗凝方式。

Objective To compare the impact of two anticoagulants （ EDTA-3K and heparin lithium ） on blood physiological parameters in miniature pigs .Methods Blood samples from 22 adult miniature pigs were collected and tested for physiological values using a MEK-7222K hematology analyzer .Differences in the values determined by using the two anticoagulants were analyzed .Results There were significant differences in values of some blood physiological parameters determined by the two anticoagulant groups , such as MON,MCV,MCHC,PLT,PCT,MPV,LYM,EOS,BAS, LYM%,MON%,EOS% and BAS%.There were significant differences in the latter 12 indexes （ P〈0.01 ） except for MON （P〈0.05）.Meanwhile, there were sex differences in values of 7 blood physiological indexes determined by EDTA-3K：PLT （P〈0.05）, NEUT （P〈0.05）,NEUT% （P〈0.05）,MON （P〈0.01）,EOS （P〈0.01）,MON% （P〈0.01） and EOS%（P〈0.01）.There were significant differences in 8 indexes determined by heparin lithium：PDW （P〈0.05）, NEUT （P〈0.05）, LYM%（P〈0.05）, MON （P〈0.01）, EOS （P〈0.01）, MON% （P〈0.01）, NEUT%（P〈0.01） and EOS%（P〈0.01）.Conclusion It is essential to choose proper anticoagulant before starting relevant tests, because there are significant differences in leukocyte and platelet counts in miniature pigs , determined with different anticoagulants .