BALB/c突变卷毛小鼠皮肤组织结构的分析

Histological analysis of the skin in BALB/c mutant mice with curly hair

目的探讨BALB/c突变卷毛小鼠的皮肤组织结构与正常BALB/c小鼠之间是否存在突变差异。方法选择10 d、21 d、42 d和63 d四个不同日龄组的BALB/c突变卷毛小鼠和正常BALB/c小鼠每组各10只,观察两组小鼠被毛外观差异情况,取两小块皮肤组织,直接镜检毛囊结构和皮肤组织病理学检测。结果 BALB/c突变卷毛小鼠生长至10 d时被毛弯曲,能够与同日龄的正常小鼠加以鉴别。镜检观察21 d、42 d和63 d的BALB/c突变卷毛小鼠毛囊中毛发根数明显少于正常小鼠,被毛稀疏且毛发卷曲明显。皮肤组织病理学检测结果显示四个不同日龄组的BALB/c突变卷毛小鼠的毛囊数均显著少于正常小鼠。63 d的BALB/c突变卷毛小鼠处于生长期的毛囊中可见毛发弯曲的现象。结论 BALB/c突变卷毛小鼠的毛囊和皮肤组织结构与正常小鼠存在明显差异,说明其皮肤组织结构也发生了改变,这为今后研究突变卷毛小鼠的突变基因和模型应用提供了理论参考。

Objective To investigate if there are differences in the skin tissue structure between BALB /c mutant mice with curly hair and normal BALB/c mice.Methods 10-d,21-d,42-d and 63-d old groups of BALB/c mutant curly mice and normal BALB/c mice were used in this study , 10 mice in each group .The gross appearance of the mice were observed.Hair follicle and skin structure were examined and compared by histopathology .Results Clothing hair of the BALB/c mutant curly mice became bent at 10 day after birth ,and could be distinguished from normal mice of the same age . Histological observation showed that the number of hair follicles and hair roots of BALB /c mutant curly mice was significantly less than that of normal mice , and the sparse coat and hair curling characteristics were obviously kept to 21 d, 42 d and 63 d of age.Histopathological examination revealed that the number of hair follicles in the mice in the four age groups of BALB/c mutant curly mice was also significantly less than that of normal mice .At 63 days of age , bending curly hair growing in hair follicles were visible in the BALB/c mutant curly mice .Conclusion Hair follicles and the skin tissue structure in the BALB/c mutant curly mice and normal mice showed distinct differences .These results demonstrate that the skin structure of mutant mice is also changed , which can provide a theoretical reference for future research on the mouse curly hair mutant gene and model application .