检测α-胞衬蛋白抗体对诊断风湿病的临床价值

Detection of Alpha Ccells Lining Protein Antibodies on the Clinical Value in the Diagnosis of Rheumatism

目的研究两种α-胞衬蛋白(αfodrin)抗体在原发性干燥综合征(pSS)等风湿病中的意义。方法选择本院门诊和住院患者256例,其中包括pSS 30例、类风湿关节炎(RA)53例、系统性红斑狼疮(SLE)36例,其他风湿病137例。以酶联免疫吸附法(ELISA)测定其血清αfodrin抗体IgG、IgA型,以免疫印迹法测定其抗SSA、SSB抗体。结果 pSS组、RA组、SLE组IgG型αfodrin抗体测定值分别为(10.87±7.44)u/mL、(8.21±4.02)u/mL、(6.70±1.50)u/mL;IgA型αfodrin抗体测定值分别为(12.72±15.52)u/mL、(6.60±2.36)u/mL、(6.55±1.73)u/mL;αfodrin抗体IgG、IgA型诊断pSS的敏感性分别为36.7%、33.3%;特异性分别为87.6%、92.1%。结论本研究检测的αfodrin抗体IgA型诊断pSS的特异性较高,与抗SSA、SSB联合检测,可以提高pSS的诊断水平,但其敏感性较低,可能与检测方法和试剂有关。

Objective To study the two alpha cells lining protein antibodies in primary sjogren′s syndrome and so on the meaning of the rheumatism.Methods Our hospital outpatient and hospitalized patients 256 cases, including 30 ca-ses of pSS, rheumatoid arthritis （RA） 53 cases, systemic lupus erythematosus36 cases, other 137 cases of rheuma-tism.By the method of enzyme -linked immunosorbent （ELISA） determination of its serum alpha fodrin antibody IgG , IgA, with western blot method to measure its anti SSA , SSB antibody.Results The pSS, RA and SLE group type IgG antibody of alpha fodrin measurements （ 10.87 ±7.44）u/mL, respectively, （ 8.21 ±4.02）u/mL, （ 6.70 ±1.50）u/mL; IgA alpha fodrin antibody measurements respectively （12.72 ±15.52）u/mL, （6.60 ±2.36）u/mL, （6.55±1.73）u/mL;Alpha fodrin antibody IgG, IgA, the sensitivity of diagnosis of pSS are 36.7%and 36.7%respec-tively;Specificity of 87.6%, 92.1%respectively.Conclusions This study to detect alpha fodrin antibody specificity of diagnosis of pSS IgA is higher , and anti SSA , SSB joint detection , can improve the diagnostic level of pSS , but its sensi-tivity is low, may be associated with testing methods and reagents.