植物生长调节剂对川麦冬总皂苷和总多糖含量的影响研究

Study of the influence of Plant Growth Regulators on Ophiopogon japonicus's Total Saponins and Total Polysaccharide Content

目的:研究植物生长调节剂对川麦冬药材总皂苷和总多糖含量的影响,为川麦冬规范化栽培提供理论依据。方法:应用紫外可见分光光度法对施用不同植物生长调节剂的川麦冬药材进行总皂苷和总多糖含量的测定。结果:低、中剂量膨大素对川麦冬总皂苷的积累有明显的促进作用;多效唑单用或与膨大素合用对总皂苷的积累均表现出与剂量呈正相关的不同程度的抑制作用。膨大素对川麦冬总多糖的生成与积累有显著的抑制作用;多效唑对川麦冬总多糖的生成与积累有显著的促进作用,作用与剂量呈负相关;两药合用时,产生不对称的拮抗作用,分段施用比混合施用的拮抗作用更明显。结论:植物生长调节剂对川麦冬总皂苷和总多糖含量有一定的影响,在川麦冬生产过程中,应严格控制植物生长调节剂的使用,以保证药材的临床疗效。

Objective： To provide a theoretical basis for standardized cultivation for Ophiopogon japonicus,we studied the influence of plant growth regulators on O. japonicus＇ s total saponins and total polysaccharide content. Method： Use the UV-visible spectrophotometric to determine the total saponins and total polysaccharide content in different plant growth regulators conducted on O. japonicus. Result： Low and medium dose of CPPU significantly promotes the accumulation of total saponins in O. japonicus; MET alone or in combination with the CPPU shows different degrees of inhibition which is positive correlated with dose. CPPU shows a significant inhibition effect on O. japonicus＇ s total polysaccharides generation and accumulation; MET shows a significant promoting effect on O. japonicus＇ s total polysaccharides generation and accumulation which is negative correlated with dose; the combined use of CPPU and MET results in asymmetry antagonism. The antagonism is more notable in segmented administration than in the hybrid administration. Conclusion： Plant growth regulators influence the total saponins and total polysaccharides content. In the O. japonicus production process,the use of plant growth regulators should be strictly controlled to ensure the clinical efficacy of medicines.