西兰花叶中硫代葡萄糖苷酸水解产物的体外和体内抗菌试验

In vitro and in vivo antibacterial test on the glucosinolate acid hydrolyzate of broccoli leaves

采用琼脂平板打孔法和试管两倍稀释法,检测了西兰花叶中硫苷酸水解产物(ITCS)对伤寒沙门氏菌、金黄色葡萄球菌、大肠杆菌、枯草芽孢杆菌的敏感性及最小抑菌浓度(MIC);并以金黄色葡萄球菌和伤寒沙门氏菌建立感染小鼠模型,观察其体内的抗菌效果.结果表明:随着硫苷酸水解产物(ITCS)的稀释抑菌效果逐渐降低、呈现剂量效应;同一浓度点ITCS的抑菌效果,其大小顺序依次是枯草芽孢杆菌>金黄色葡萄球菌>伤寒沙门氏菌>埃希大肠杆菌;伤寒沙门氏菌和大肠杆菌的西兰花叶粉硫苷水解产物(ITCS)最小抑菌浓度(MIC)均为1/40,即为0.234μmol/mL;而对于金黄色葡萄球菌、枯草芽孢杆菌硫苷水解产物(ITCS)最小抑菌浓度(MIC)均为1/80,即为0.117μmol/mL;ITCS对感染金黄色葡萄球菌和伤寒沙门氏菌的小鼠具有一定的保护作用,其中对金黄色葡萄球菌感染小鼠体内的抗菌效果优于伤寒沙门氏菌.试验表明,ITCS具有一定的体外和体内的抗菌作用.

This experiment was conducted to test the sensitivity and minimum inhibitory concentration （MIC）of the glucosinolate acid hydrolyzate （ITCS）in broccoli leaves on Salmonella,S.aureus,E.coli and Bacillus subtilis by the agar plate punching and tube double dilution methods.Antibacterial effects were al-so observed by mice infected with Staphylococcusaureus and Salmonellainvivo.The results showed：With dilution of ITCS,antibacterial effects reduced gradually;the rank of the same ITCS concentration point for antibacterial effects was：Bacillus subtilis 〉 Staphylococcus aureus 〉 Salmonella typhi 〉 Escherichia coli.The MIC of ITCS for E.coli,Salmonella,Staphylococcus aureus and Bacillus subtilis was 1/40 （0.234μmol/mL）,1/40 （0.234μmol/mL）,1/80 （0.117μmol/ml）and 1/80 （0.117μmol/ml）,respective-ly.It showed that the ITCS had certain effects on protecting mice infected with Staphylococcus aureus and Salmonella,and better protection obtained for resisting Staphylococcus aureus.The experimental results showed that ITCS had certain antibacterial activity after invitro and invivo test.