海南普通野生稻STK类抗病基因同源序列的分离与分析

Cloning and analysis of STK resistance gene analogues from Hainan common wild rice(Oryza rufipogon Griff)

根据植物STK抗病基因的保守区域设计简并引物,以海南普通野生稻基因组DNA为模板进行PCR扩增,通过T/A克隆、测序和序列分析,共获得5条具有连续ORF的STK抗病基因类似物（RGAs）序列,核苷酸序列间的相似性系数为37.82%99.25%,相应氨基酸序列间的相似性系数为31.28%98.86%。氨基酸序列结构分析表明,它们都具有STK保守结构域,与已克隆的STK类抗病基因有不同程度的相似性,为进一步克隆海南普通野生稻中的STK类抗病基因提供依据。

Degenerate primers based on conserved motif of the serine-threonine kinase（STK） region from the cloned plant disease resistance genes were used to isolate resistance gene analogues（RGAs） from genomic DNA of Hainan common wild rice（Oryza rufipogon Griff）. After T/A cloning, sequencing and analyzing by alignment, five RGAs with uninterrupted open reading frames（ORFs） were obtained. Sequence identity among the five RGAs nucleotide sequences ranged from 17.96% to 98.86%, while the five RGAs deduced amino acid sequences showed identity ranged from 37.82% to 99.25%. The deduced amino acid sequences of RGAs contained STK motifs and had different homologies to STK resistance genes that had cloned. These provided the basis for further study on cloning STK resistance gene from Hainan common wild rice.