甘肃道地中药大黄、黄芩对多重耐药菌的抑菌活性

Antibacterial Activity of Gansu Genuine Herbs DaHuang and HuangQin on Multiple Resistant Bacteria

目的:观察甘肃道地中药材大黄、黄芩的体外抑菌活性,为临床中药治疗耐药菌感染提供参考。方法:采用煎煮法制备大黄、黄芩中药原液,用肉汤稀释法测定两种药液对产超广谱β-内酰胺酶(ESBLs)大肠埃希菌和耐甲氧西林金黄色葡萄球菌(MRSA)的最小抑菌浓度。结果:大黄对产超广谱β-内酰胺酶(ESBLs)大肠杆菌和耐甲氧西林金黄色葡萄球菌(MRSA)的最小抑菌浓度分别为250 mg/mL和125 mg/mL。黄芩对耐甲氧西林金黄色葡萄球菌(MRSA)的最小抑菌浓度为250 mg/mL;对产超广谱β-内酰胺酶(ESBLs)大肠杆菌未见明显抑菌作用。结论:大黄、黄芩对临床常见的多重耐药菌有不同程度的抑菌活性,且大黄的抑菌活性优于黄芩;大黄对金黄色葡萄球菌抑菌活性优于大肠埃希菌。

Objective： To provide reference for herbs in treating drug resistant bacteria infection by observing antibacterial activity of Gansu genuine herbs DaHuang （Rheum palmatum L.） and HuangOin （Seutellaria Baicalensis） in vitro. Methods： The decoctions of Dattuang and HuangQin were prepared, the minimum antibacterial concentrations of two kinds of decoctions for extended spectrumfl lactamases（ESBLs） E.coli and methicillin-resistant staphylococcus aureus （MRSA） were detected with broth dilution method. Results： The minimum antibacterial concentrations of Daltuang for producting ESBLs E.coli and MRSA were 250mg/mL and 125 mg/mL. The minimum antibacterial concentrations of HuangQin for producting MRSA were 250mg/mL, and it didn＇t show notable antibacterial function to ESBLs E.coli. Conclusion： Dattuang and HuangQin show different degrees of antibacterial activity to common multiple resistant bacteria, and antibacterial activity of DaHuang is superior to that of HuangQin; antibacterial activity of DaHuang to MRSA is superior to that of ESBLs E.coli.