‘北林雄株1号’和‘北林雄株2号’叶片再生体系的建立

Establishment of Leaf-explant Regeneration System in Vitro of Triploid Hybrids of White Poplar‘Beilixiongzhu 1'and‘Beilinxiongzhu 2'

为了解决白杨杂种三倍体‘北林雄株1号’和‘北林雄株2号’无性繁殖困难问题,以白杨杂种三倍体‘北林雄株1号’和‘北林雄株2号’的半木质化嫩枝为材料,对外植体消毒方法以及不同激素组合对叶片诱导不定芽的效果进行研究。结果表明:(1)‘北林雄株1号’和‘北林雄株2号’启动培养最佳的消毒处理方法为20%浓度的84消毒液处理20 min;(2)激素6-BA以及TDZ对‘北林雄株1号’叶片不定芽再生率以及再生系数的影响显著,NAA对叶片不定芽再生率影响不显著,对再生系数的影响极显著,筛选出‘北林雄株1号’叶片再生的适宜培养基为MS+0.2 mg/L 6-BA+0.1 mg/L NAA+0.005 mg/L TDZ;(3)激素6-BA、NAA以及ZT对‘北林雄株2号’叶片再生率和再生系数的影响均达到了显著水平,筛选出‘北林雄株2号’叶片再生的适宜培养基为MS+1.0 mg/L 6-BA+0.05 mg/L NAA+1.2 mg/L ZT。‘北林雄株1号’与‘北林雄株2号’高效叶片再生技术体系建立,为该品种的规模化无性繁殖以及后续的基因工程育种等研究提供了技术基础。

To solve the difficulties in vegetative propagation of triploid hybrids of white poplar‘Beilinxiongzhu 1＇and‘Beilinxiongzhu 2＇, the effects on disinfection method and adventitious buds from different hormones combination were studied by taking semi-woody shoots came from triploid hybrids of white poplar ‘Beilinxiongzhu 1＇and‘Beilinxiongzhu 2＇as explants. The results indicated that：（1） the suitable method for disinfection of the two triploid hybrids was to treat for 20 minutes by 20% 84 disinfectant.（2）6-BA and TDZ had significant effects on regeneration rate and coefficient of the adventitious buds. However, NAA had insignificant effect on regeneration rate of the adventitious buds, but significant effect on coefficient of the adventitious buds. The optimum medium for the regeneration of adventitious bud from leaves in ‘Beilinxiongzhu 1＇was MS＋ 0.2 mg/L 6-BA＋ 0.1 mg/L NAA＋ 0.005 mg/L TDZ.（3） Significant effects on regeneration rate and coefficient of the adventitious buds of 6-BA, NAA, and TDZ were observed in the triploid hybrid‘Beilinxiongzhu 2＇. The optimum medium for effective inducing adventitious buds in vitro leaves was MS＋ 1.0 mg/L 6-BA＋ 0.05 mg/L NAA＋ 1.2 mg/L ZT. The rapid propagation systems of leaf explants in the two triploid hybrids were successfully established, which provided a platform for large-scale vegetative propagationand further genetic transformation of‘Beilinxiongzhu 1＇and‘Beilinxiongzhu 2＇.