中草药饲料添加剂“连藤”合剂质量标准的初步研究

Preliminary Research on the Quality Standards Chinese Herbal Medicine Feed Additive Compound Preparation “Lianteng”

选择中草药连翘、鸡血藤等配伍组成“连藤”合剂,建立“连藤”合剂的质量标准.采用薄层色谱法对处方中的连翘苷和芒柄花素进行鉴别,并采用高效液相色谱法对其含量进行测定,色谱条件为phenomenex luna C18（250 mm×215;4.60 mm,5μm）色谱柱,柱温25 ℃,乙腈-0.3％磷酸溶液为流动相梯度洗脱,流速为1mL/min,检测波长为254 nm和277 nm.结果表明,薄层鉴别的色谱分离良好,斑点清晰;连翘苷在进样量为25～100 μg范围内线性关系良好（R^2=0.999 5）,平均回收率为96.40％（n=6）;芒柄花素在进样量为12～120μg范围内线性关系良好（R^2=0.999 8）,平均回收率为95.13％（n=6）.该方法专属性强,且准确、快速、结果可靠,适用于“连藤”合剂的质量控制.

The compound preparation ＂Lianteng＂ was composed of Chinese herbal medicine Forsythia suspensa Vahl and Caulis spatholobi so as to establish the quality standards of compound preparation ＂Lianteng＂. Phillyrin and formononetin in the Chinese medicine preparation were identified by using thin-layer chromatography. And its content was determined by using highperformance liquid chromatography. The chromatographic conditions were as follows： phenomenex luna C 18 （250 mm×4.60 mm, 5 μm）chromatographic column, column temperature of 25 ℃, eluting with methyl cyanide-0.3% phosphoric acid solution as mobile phase, flow rate of I mL/min, the detection wavelength of 254 nm and 277 nm. The results showed that phillyrin and formononetin were separated by using thin-layer chromatography with clear spots. There was a good linear relation of phillyrin at the sample amount of 25-100 μg （R2= 0.999 5 ）and the average recovery was 96.40% （n=6）. There was a good linear relation of formononetin at the sample amount of 12-120 μg （R2=0.999 8）and the average recovery was 95.13% （n=6）.The methods of HPLC and TLC were rapid, acute and repeatable, which was suitable for the quality control of compound preparation ＂Lianteng＂.