摘要
Increasing awareness concerning food safety problems has been driving the search for simple and efficient biochemical analytical methods.In this paper,we develop a portable electro-acoustic biosensor based on a film bulk acoustic resonator for the detection of pesticide residues in agricultural products.A shear mode ZnO film bulk acoustic resonator with a micro-machining structure was fabricated as a mass-sensitive transducer for the real-time detection of antibody-antigen reactions in liquids.In order to obtain an ultra-low detection level,the artificial antigens were immobilized on the sensing surface of the resonator to employ a competitive format for the immunoassays.The competitive immunoreactions can be observed clearly through monitoring the frequency changes.The presence of pesticides was detected through the diminution of the frequency shift compared with the level without pesticides.The limit of detection for carbaryl(a widely used pesticide for vegetables and crops)is2?10-10 M.The proposed device represents a potential alternative to the complex optical systems and electrochemical methods that are currently being used,and represents a significant opportunity in terms of simplicity of use and portability for on-site food safety testing.
Increasing awareness concerning food safety problems has been driving the search for simple and efficient bio-chemical analytical methods. In this paper, we develop a portable electro-acoustic biosensor based on a film bulk acoustic reso-nator for the detection of pesticide residues in agricultural products. A shear mode ZnO film bulk acoustic resonator with a mi-cro-machining structure was fabricated as a mass-sensitive transducer for the real-time detection of antibody-antigen reactions in liquids. In order to obtain an ultra-low detection level, the artificial antigens were immobilized on the sensing surface of the resonator to employ a competitive format for the immunoassays. The competitive immunoreactions can be observed clearly through monitoring the frequency changes. The presence of pesticides was detected through the diminution of the frequency shift compared with the level without pesticides. The limit of detection for carbaryl （a widely used pesticide for vegetables and crops） is 2′10-10 M. The proposed device represents a potential alternative to the complex optical systems and electrochemical methods that are currently being used, and represents a significant opportunity in terms of simplicity of use and portability for on-site food safety testing.
基金
supported by the National Science Foundation of China(No.61101027)
Qingdao Science and Technology Program(No.12-1-4-6-(8)-jch)
Shangdong Provincial Young and Middle-Aged Scientists Research Awards Fund(No.BS2012DX032)
Shandong Provincial Natural Science Foundation(No.ZR2013FM018)
SDUST Research Funds(Nos.2011KYJQ101 and 2012KYTD103)
