环氧化酶2基因多态性与急性冠状动脉综合征的关联研究

Association between COX-2 gene polymorphism and acute coronary syndrome

目的探讨环氧化酶2（COX-2）基因-1195G＆gt；A和-765G＆gt；C位点与中国汉族人群急性冠状动脉综合征（ACS）的相关性。方法选择ACS患者250例（ACS组）,同期选取健康体检者266例（对照组）。采用扩增阻碍突变系统结合TaqMan探针的实时荧光定量PCR方法对COX-2基因多态性位点（-1195G＆gt；A和-765G＆gt；C）进行基因分型。多因素logistic回归分析各多态性位点与ACS的相关性。结果在男性中,ACS组-1195G＆gt；A基因型及等位基因频率与对照组比较,差异有统计学意义（GG：23.4%vs 33.8%,GA：50.6%vs 49.4%,AA：25.5%vs 16.9%,P=0.049；G：48.7%vs 58.4%,A：51.3%vs 41.6%,P=0.014）；与-1195G＆gt；A位点GG基因型比较,AA＋GA基因型发生ACS的风险更高（P=0.042）,行多因素分析校正相关危险因素后,差异有统计学意义（OR=1.971,95%CI：1.130～3.438,P=0.017）。在女性中,ACS组-1195G＆gt；A位点基因型及等位基因频率与对照组比较,差异无统计学意义（P〉0.05）。单体型分析提示,在男性中,ACS组A-1195-765-G频率显著高于对照组,差异有统计学意义（P=0.012）,而在女性中,ACS组A-1195-765-G频率差异无统计学意义（P〉0.05）。结论在中国汉族人群中,COX-2基因-1195G〉A基因多态性以及单体型A-1195-765-G与男性ACS有关,与女性无关。

Objective To study the association between COX-2 gene polymorphism at -1195G〉A and -765G〉C and acute coronary syndrome （ACS） in Chinese Han people. Methods Two hun- dred and fifty ACS patients served as an ACS group and 266 subjects undergoing physical exami- nation served as a control group in this study. Their COX-2 gene polymorphism at -l195G〉A and -765G〉C was genotyped by RT-PCR. Association between COX-2 gene polymorphism at -1195G〉 A and -765G〉C and ACS was analyzed by multivariate logistic regression analysis and the haplo- type was analyzed using the SHEsis software. Results The frequency of -l195G〉A and allelic gene was significantly higher in male ACS patients than in controls （P=0. 049,P=0. 014）. The risk to develop ACS was higher in GAand AA genotype than in GG genotype at -1195G〉A （P= 0. 042）. Multivariate logistic regression analysis showed that the risk factors for ACS were signifi- cantly different （OR= 1. 971,95〉CI. i. 130--3. 438, P= 0. 017）. No significant difference was found in frequency of -1195G〉A and allelic gene between female ACS patients and controls （P〉0.05）. Haplotype analysis displayed that the frequency of -l195G〉A and -765G〉C was signifi- cantly higher in male ACS patients than in controls （P=0. 012） with no significant difference in female ACS patients （P〉0.05）. Conclusion COX-2 gene polymorphism at -1195G〉A and -765 G〉C is associated with ACS in Chinese Han males but not in Chinese Han females.