糖尿病性勃起障碍大鼠阴茎海绵体平滑肌组织形态学及α-SMA表达量的变化

Changes in the histology morphology and the expression of α-SMA in smooth muscle tissue of corpus cavernosum of rats with diabetic erectile dysfunction

目的:通过大鼠实验总结糖尿病性勃起障碍阴茎海绵体平滑肌组织形态学和α-SMA表达变化。方法:将20只正常大鼠分为观察组(12只)和对照组(8只)。观察组为糖尿病性勃起障碍大鼠,对照组为正常大鼠。取两组阴茎海绵体组织,采用Masson三色法和免疫荧光化学染色法测定平滑肌/胶原纤维相对含量和α-SMA表达。结果:对照组阴茎勃起率为100%,观察组阴茎勃起率为8.33%。观察组在2.5、5.0、7.5V电刺激下Max ICP/MAP比值均显著低于对照组(P<0.05)。观察组平滑肌/胶原纤维比值为0.16±0.04,α-SMA表达为0.25±0.17,均显著低于对照组,有统计学差异(P<0.05)。结论:糖尿病性勃起障碍大鼠的平滑肌数量减少,胶原纤维明显增长,α-SMA表达受抑。上述情况可能是勃起障碍的主要原因之一。

Objectives： To summarize changes in the histology morphology and α- SMA expression of smooth muscle in corpus cavernosum of rats with diabetic erectile dysfunction. Method： Twenty rats were selected and divided into observation group and control group,12 rats with diabetic erectile dysfunction as the observation group and 8 normal rats as the control group. The penile tissue of rats in the two groups were collected and then stained by Masson staining and immunofluorescence to determinate the expression of smooth muscle / relative content of collagen and α- SMA. Results： The penile erection rate of the control group was 100% while that of the observation group was 8. 33%. Given 2. 5,5,7. 5V electrical stimulation respectively,the Max ICP / MAP ratio of the observation group were significantly lower than those in the control group（ P〈0. 05）. Muscle / collagen ratio andα- SMA expression level in the observation group was（ 0. 16 ± 0. 04） and（ 0. 25 ± 0. 17）,significantly lower than the control group（ P 〈0. 05）. Conclusions： For rats with diabetic erectile dysfunction,the amount of smooth muscle significantly decrease,the collagen fibers significantly increase,and the expression of α- SMA is inhibited, which maybe the possible causes of erectile dysfunction.