大孔吸附树脂分离花生种皮原花青素的研究

Separation of proanthocyanidin from peanut seed coat with macroporous absorption resin

研究比较了4种大孔吸附树脂对花生种皮原花青素分离的影响,筛选出最适的分离原花青素大孔吸附树脂AB-8型,并确定了AB-8型大孔吸附树脂的最佳工艺条件:样品浓度为2.5 mg/mL,上样流速为0.5 mL/min。采用20%、30%、50%浓度的乙醇溶液进行洗脱,得到3个不同的原花青素洗脱级分别为PSPP1、PSPP2、PSPP3,其中原花青素的含量分别为75.26%、84.50%和90.18%。通过薄层层析分析得知,提纯的原花青素样液有三条谱带,初步推断为单体、低聚体混合物及高聚体。

The effect of four different macroporous absorption resins on the extraction of proanthocyanidinfrom peanut seed coat was compared. The optimum macroporous adsorption resin was screened out whichwas AB -8. The optimal conditions were： concentration of the sample was 2.5 mg/mL, loading flow ratewas 0.5 mL/min. The proanthocyanidin was eluted with ethanol concentration of 20%, 30%, and 50%,and three levels of the liquid, PSPP1, PSPP2, and PSPP3, were obtained with the proanthocyanidin con-tent 75.26%, 84.50%, and 90.18% respectively. The thin layer chromatography analysis showed that thepurified liquid had three bands, initial inference as monomer, mixture of oligomers, and high polymers.