复方丹参滴丸对高血脂症小鼠血小板功能的影响

Effect of Fufang Danshen Dripping Pills on Platelets Function of Hyperlipidemic Mice

【目的】观察复方丹参滴丸对高血脂症小鼠血小板功能的影响并探讨其作用机制。【方法】选用SPF级昆明种小鼠,采用喂饲高脂饲料复制高血脂症小鼠模型,动物随机分为5组:正常对照组,模型组,复方丹参滴丸高、中、低剂量组(剂量分别为400、160、40 mg·kg-1·d-1),给药2周后采血,测定各组小鼠血脂水平,采用流式细胞仪检测各组小鼠的血小板功能。【结果】复方丹参滴丸对高血脂症小鼠的体质量无显著影响,复方丹参滴丸高剂量组可以显著降低高血脂症模型小鼠的总胆固醇水平(P<0.01)。高血脂症模型组小鼠活化血小板比例、单核细胞—血小板聚集率、中性粒细胞—血小板聚集率均显著高于正常对照组(P<0.01),不同剂量复方丹参滴丸组均可显著减少高血脂症小鼠活化血小板比例(P<0.01),复方丹参滴丸高剂量组可显著减少高血脂症小鼠单核细胞—血小板聚集率和中性粒细胞—血小板聚集率(P<0.05或P<0.01),复方丹参滴丸中剂量组可显著减少中性粒细胞—血小板聚集率(P<0.01)。【结论】复方丹参滴丸可抑制高血脂症小鼠的血小板活化,其作用机理与抑制单核细胞—血小板聚集和中性粒细胞—血小板聚集有关。

Objective To study the effect of Fufang Danshen Dripping Pills（FDDP） on the platelet function of hyperlipidemic mice and to explore its mechanism. Methods Specific-pathogen free Kuming mice were randomly divided into 5 groups, namely normal control group, model group, and high-, middle- and low-dose FDDP groups（ in the dosage of 400, 160, 40 mg·kg-1·d-1, respectively）. The hyperlipidemic mice model wasestablished by feeding high fat diet. After administration for 2 weeks, the mice blood was sampled for the determination of blood lipids and platelet function. Results FDDP had no effect on body weight of hyperlipidemic mice. High-dose FDDP significantly reduced the serum total cholesterol level of hyperlipidemic mice（P0.01）. The percentage of activated platelet, monocyte-platelet aggregation rate, neutrophil-platelet aggregation rate in hyperlipidemic mice were significantly higher than those of the normal control group（ P 〈0.01）, and then the activated platelet percentage was decreased after intervention with FDDP（ P 〈0.01）. High-dose FDDP significantly decreased monocyte-platelet aggregation rate and neutrophil-platelet aggregation rate（P〈0.05 or P〈 0.01）, and middle-dose FDDP significantly decreased neutrophil-platelet aggregation rate（P〈0.01）. Conclusion FDDP have obvious effect on inhibiting platelet activation, and the mechanism is probably related with inhibiting the monocyte-platelet aggregation and neutrophil-platelet aggregation.