康脑液对脑缺血再灌注动物血管新生的影响

Effect of Kangnaoye on angiogenesis in rats after cerebral ischemic reperfusion

目的观察康脑液对大鼠局灶脑缺血再灌注损伤后血管新生的影响。方法将雄性SD大鼠随机分为假手术组、模型组、尼莫地平组(1 mg·kg-1·d-1)及高、中、低剂量(24,12,6 g·kg-1·d-1)康脑液组,线栓法制备大鼠脑缺血再灌注模型。于缺血后2 h再灌注0.5,3,7 d后,处死大鼠,用免疫组织化学法观察脑缺血区微血管密度(MVD)的变化及脑组织血管内皮生长因子(VECF)和肝细胞生长因子(HGF)的表达情况;同时分别于再灌注后2,24,48 h评价动物神经功能。结果与模型组相比,各剂量康脑液组与尼莫地平组的神经功能均明显改善,MVD显著升高,VEGF和HGF蛋白表达明显上调(P<0.05或P<0.01),其中以中剂量康脑液组最为显著。结论康脑液增强大鼠局灶性脑缺血后VEGF及HGF的表达,是其抗脑缺血的可能作用机制之一。

Objective To observe the effect of Kangnaoye on angiogene- sis in rats after focal cerebral ischemia reperfusion. Methods The male SD rats were randomly divided into 6 groups： sham operation group, model group, Kangnaoye high, middle and low dosages groups （24,12,6 g. kg-l .d-1）, andnimodipinegroup（1 rag. kg-I . d-l）. The mod- els of cerebral ischemia reperfusion were established in rats by methods of middle cerebral artery occlusion （MCAO）. Immunohistochemical method was used to observe the change of the microvessel density （ MVD ） after cerebral ischemic and the expression of vascular endothelial growth （ VEG）, HGF and CD31 protein of rats which were killed at the O. 5, 3, and 7 d of reperfusion injury respectively 2 h after cerebral ischemia. The nervous function deficit scores were evaluated at the 2, 24, and 48 h af- ter reperfusion. Results Compared with model group, the neurological behavior performance in Nimodipine and Kangnaoye treatment groups were significantly improved, with an increased number of MVD, and en- hanced vascular endothelial growth factor（VEGF） and hepatocyte growth factor（HGF） protein levels （ P 〈 0. 05 or P 〈 O. O1 ）, especially in the Kangnaoye middle dosage group. Conclusion Kangnaoye has neuropro- tective effect against focal cerebral ischemic injury by improving the ex- pression of VEGF and HGF, whicla＇＇is one of possible anti - ischemic mechanisms.