稀有D--表型产妇血清学检测及家系调查

Serological test and family survey of puerperal women with rare D--phenotype

目的:检测分析Rh缺失型D--的血型血清学特性和遗传背景。方法:使用特异性抗体检测先证者红细胞表面的D、C、c、E和e抗原,吸收试验进一步证实D--表型;采用经典抗球蛋白法鉴定先证者血清中的抗体特异性及效价;检测家庭成员的Rh表型,分析D--的家系遗传。结果:先证者为D--表型,血清中存在针对高频抗原R17的抗体,效价为512,家庭成员中先证者的妹妹为D--,父亲、母亲、姐姐及2个孩子均为D--杂合子。结论:D--表型存在家族遗传特性,亲属的Rh表型筛查有助于D--血液的获取。

Objective： To detect and analyze the serological characteristics and genetic background of puerperal women with rare Rh D - - phenotype. Methods ： Specific antibody was used to detect D, C, c, E, e antigens on the surface of red blood cells of proposi- tus, D - - phenotype was confirmed by adsorption test. Antibody specificity and titer in serum of propositus was detected by classic indirect antiglobulin test. Rh phenotypes were detected among the family members of propositus, D - - family heredity was analyzed. Results ： The Rh phenotype of propositus was confirmed as D - - phenotype. Antibody targeting to high frequency Rhl7 antigen existed in serum, and the titer was 512. Family study revealed that propositus＇younger sister was D - - phenotype, five family members （father, mother, older sister and two children） were D - - heterozygous. Conclusion： Family genetic characteristics of D- - phenotype is helpful to obtain D -blood from family members.