NOD2表达上调对人早期妊娠蜕膜基质细胞生物学行为的影响

Effect of up-regulation of NOD2 on biological behavior of human decidual stromal cells during the first trimester of pregnancy

目的:探讨胞内模式识别受体NOD2表达上调对于人早期妊娠蜕膜基质细胞基本生物学行为的影响。方法:分离培养人早期妊娠蜕膜基质细胞运用CCK8试剂盒、Transwell侵袭小室、流式细胞术等分别检测接受NOD2配体处理后细胞增殖、侵袭和凋亡能力。结果:蜕膜基质细胞接受相应配体刺激后NOD2 mRNA及蛋白表达水平上调,抑制细胞侵袭能力促进细胞凋亡,但是对细胞增殖能力无明显影响。结论:NOD2过度激活损伤蜕膜组织,可能导致不良妊娠结局。

Objective： To explore the effect of NOD2 on biological behavior of human decidual stromal cells during the first trimes- ter of pregnancy. Methods： Human decidual stromal cells during the first trimester of pregnancy were isolated and cultured, then CCK8 kit, Trans - well assay and flow cytometry were used to detect proliferation ability, invasive ability and apoptotic ability of human decidual stromal cells after treated with NOD2 ligand. Results： After stimulated with NOD2 ligand, the expression levels of NOD2 mRNA and protein in human decidual stromal cells were up - regulated, their invasive ability was inhibited and apoptosis was promoted, but it had no significant impact on cell proliferation. Conclusion： Excessive activation of NOD2 can injury decidual tissue and induce adverse pregnancy outcome.